摘要

       本研究调查了在堆肥过程中添加三个水平（0%、5% 和 10% w/w）的煤气化炉渣 (CGS) 对抗生素抗性基因 (ARG) 和细菌群落的影响。 CGS 的添加有效地促进了 ARG 和移动遗传元件 (MGE) 的去除，显着降低了猪粪堆肥产品中 5/11 ARG 和三种 MGE 的相对丰度。 此外，与 0% CGS 相比，添加 10% CGS 时 ARGs 和 intI1 的富集较低。 不同处理下菌群根据堆肥时间分布。 冗余分析表明，细菌群落演替和 MGE 介导的水平基因转移在 ARG 的变异中起重要作用。 网络分析表明 ARG 和 MGE 与特定微生物共存。 因此，10% CGS 可能是降低堆肥产品中 ARGs 风险的合适添加剂.

       This study investigated the effects of the addition of coal gasification slag (CGS) at three levels (0%, 5%, and 10% w/w) on antibiotic resistance genes (ARGs) and the bacterial community during composting. The addition of CGS effectively facilitated the removal of ARGs and mobile genetic elements (MGEs), where it significantly reduced the relative abundances of 5/11 ARGs and three MGEs in the swine manure composting product. In addition, the enrichment of ARGs and intI1 was lower under the addition of 10% CGS compared with 0% CGS. The bacterial community was distributed according to the composting time under different treatments. Redundancy analysis showed that bacterial community succession and MGE-mediated horizontal gene transfer played important roles in the variations in ARGs. Network analysis indicated the co-occurrence of ARGs and MGEs with specific microorganisms. Thus, 10% CGS may be a suitable additive for reducing the risks of ARGs in compost products.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418310113