摘要

       畜禽粪便是抗生素抗性基因 (ARG) 的热点。 固态厌氧消化 (SAD) 是一种高效的牲畜粪便处理方法，但在 SAD 期间 ARGs 和微生物群落的变化尚不清楚。 因此，进行了嗜热 SAD (55°C)、中温 SAD (35°C) 和液体厌氧消化 (35°C) 以研究 SAD 对 ARG、移动遗传元件和微生物群落的影响。 与液体厌氧消化相比，SAD 显着减少了至少 7/10 的 ARG 和所有考虑的移动遗传元件，其中，嗜热 SAD 产品中 ARG 的总丰度比中温 SAD 产品高 23.7%。 Firmicutes 和 Proteobacteria 是 ARGs 的主要潜在宿主，它们丰度的减少是 SAD 期间 ARGs 减少的主要原因。 该研究表明，SAD 是降低牲畜粪便中 ARGs 风险的一种很有前景的方法。 

       Livestock manure is a hotspot for antibiotic resistance genes (ARGs). Solid-state anaerobic digestion (SAD) is a high efficiency livestock manure treatment, but the changes in ARGs and microbial communities during SAD are unknown. Therefore, thermophilic SAD (55 °C), mesophilic SAD (35 °C), and liquid anaerobic digestion (35 °C) were conducted to investigate the impacts of SAD on ARGs, mobile genetic elements, and microbial communities. Compared with liquid anaerobic digestion, SAD significantly reduced at least 7/10 ARGs and all of the mobile genetic elements considered, where the total abundance of ARG was 23.7% higher in the thermophilic SAD product than the mesophilic SAD product. Firmicutes and Proteobacteria were the main potential hosts for ARGs, and their decreased abundances were mainly responsible for the reductions in ARGs during SAD. This study demonstrated that SAD is a promising method for decreasing the ARGs risk in livestock manure.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418312501