摘要

       抗生素耐药性细菌是一个新出现的全球性问题，有可能破坏现代医学的重要进展。环境可能在抗生素抗性基因 (ARG) 在环境细菌和致病细菌中的传播中发挥重要作用。污水处理厂 (WWTP) 从周围的城市环境中积累化学和生物废物，因此被视为传播和发展抗生素抗性的潜在热点。为了评估废水对流经瑞典城市的河流的影响，从 Stångån 河（邻近污水处理厂的上游和下游）收集了超过 3 个月的沉积物和水样。使用实时聚合酶链反应 (PCR) 在收集的沉积物中量化了七个 ARG 和 1 类整合子上的整合酶基因。液相色谱-质谱法用于评估样品水相中 10 种不同抗生素的丰度。结果显示污水处理厂下游的 ARG 和整合子增加。在 Stångån 河的水样中测得的抗生素浓度很低，表明地表水中没有发生 ARG 的选择。相反，下游 ARG 的增加可能归因于从 WWTP 排放的处理过的污水中存在的基因的积累。环境毒理学化学 2015；34：192-196。

       Antibiotic-resistant bacteria are an emerging global problem that threatens to undermine important advances in modern medicine. The environment is likely to play an important role in the dissemination of antibiotic-resistance genes (ARGs) among both environmental and pathogenic bacteria. Wastewater treatment plants (WWTPs) accumulate both chemical and biological waste from the surrounding urban milieu and have therefore been viewed as potential hotspots for dissemination and development of antibiotic resistance. To assess the effect of wastewater effluent on a river that flows through a Swedish city, sediment and water samples were collected from Stångån River, both upstream and downstream of an adjacent WWTP over 3 mo. Seven ARGs and the integrase gene on class 1 integrons were quantified in the collected sediment using real-time polymerase chain reaction (PCR). Liquid chromatography-mass spectrometry was used to assess the abundance of 10 different antibiotics in the water phase of the samples. The results showed an increase in ARGs and integrons downstream of the WWTP. The measured concentrations of antibiotics were low in the water samples from the Stångån River, suggesting that selection for ARGs did not occur in the surface water. Instead, the downstream increase in ARGs is likely to be attributable to accumulation of genes present in the treated effluent discharged from the WWTP. Environ Toxicol Chem 2015;34:192–196. 

https://setac.onlinelibrary.wiley.com/doi/abs/10.1002/etc.2784