发布者:抗性基因网 时间:2021-06-29 浏览量:706
摘要
在本文中,评估了粘土改良剂对抗生素抗性基因 (ARG) 命运的影响及其与家禽粪便 (PM) 堆肥中细菌多样性的关系。结果证实,4% 的粘土改良剂显着降低了 94% 的 ARG 的相对丰度 (RAs),而与其他粘土混合处理相比,较高剂量的粘土混合处理(T5 和 T6)堆肥的 ARG 百分比相对非常高.添加粘土通过改变厚壁菌门和变形菌门的整体 RA,在堆肥过程中对 ARGs 的发生和分布发挥潜在作用,并在那里宿主细菌物种。热图相关图还表明,ARGs 的减少与堆肥过程中微生物动力学和物理化学参数的改变显着相关。因此,在 PM 堆肥系统中添加 4% 的粘土可以有效地减轻 PM 堆肥中 ARGs 的更高浓度以及与其应用相关的环境风险。
In this paper, the influence of clay amendment on antibiotic resistance genes (ARGs) fate and their relation with bacterial diversity in poultry manure (PM) composting was evaluated. The results confirmed that 4% clay amendment was significantly reduce the relative abundances (RAs) 94% of ARGs, while higher dosage of clay blended treatments (T5 and T6) composts were observed relatively very high percentage of ARGs as compared to other clay blended treatments. Clay addition play potential role on the occurrence and distribution of ARGs during the composting by altering the overall RAs of Firmicutes and Proteobacteria, and there host bacterial species. The heat map correlation figure also indicated that reduction of ARGs was significantly associated with the alteration of microbial dynamics and physicochemical parameters during the composting. Therefore, the addition of 4% clay in to PM composting system could efficiently mitigate the greater concentration of ARGs in PM compost and also environmental risk associated with its application.
https://www.sciencedirect.com/science/article/abs/pii/S0960852419302111