摘要

       研究了具有 A2O-MBR 系统的全规模市政污水处理厂中五种抗生素抗性基因 (ARG)——tetG、tetW、tetX、sul1 和 intI1 的变异。进水和膜生物反应器 (MBR) 出水中五种抗性基因的浓度随着 sul1 > intI1 > tetX > tetG > tetW 而降低，并且 sul1 的丰度在统计学上高于其他三种四环素抗性基因（tetG、tetX、tet ) (p < 0.05)。春季进水中5种ARGs的浓度均高于其他季节（中位数105.81-107.32拷贝mL-1），tetW、tetX和sul1在秋季达到最低浓度（104.61-106.81拷贝mL-1） 1）。废水中ARGs的浓度在厌氧出水和缺氧出水中降低，但在好氧出水中增加，然后在MBR出水中急剧下降。 tetW、intI1和sul1的减少均与废水处理过程中16S核糖体DNA（rDNA）的减少呈显着正相关（p < 0.01）。污泥样品中 ARGs 的浓度（mg−1）随着处理过程而增加，但 5 个 ARGs 的丰度（ARGs 与 16S rDNA 的比率）从厌氧池到缺氧池到好氧池保持不变，而 MBR 中的增加比率观察到所有 ARG。

       The variation of five antibiotic resistance genes (ARGs)—tetG, tetW, tetX, sul1, and intI1—in a full-scale municipal wastewater treatment plant with A2O-MBR system was studied. The concentrations of five resistance genes both in influent and in membrane bioreactor (MBR) effluent decreased as sul1 > intI1 > tetX > tetG > tetW, and an abundance of sul1 was statistically higher than three other tetracycline resistance genes (tetG, tetW, and tetX) (p < 0.05). The concentrations of five ARGs in the influent were all higher in spring (median 105.81–107.32 copies mL−1) than they were in other seasons, and tetW, tetX, and sul1 reached its lowest concentration in autumn (104.61–106.81 copies mL−1). The concentration of ARGs in wastewater decreased in the anaerobic effluent and anoxic effluent, but increased in the aerobic effluent, and then sharply declined in the MBR effluent. The reduction of tetW, intI1, and sul1 was all significantly positively correlated with the reduction of 16S ribosomal DNA (rDNA) in the wastewater treatment process (p < 0.01). The concentration of ARGs (copies mg−1) in sludge samples increased along the treatment process, but the abundance of five ARGs (ratio of ARGs to 16S rDNA) remained the same from anaerobic to anoxic to aerobic basins, while an increment ratio in MBR was observed for all ARGs.

https://link.springer.com/article/10.1007/s11356-014-3552-x