摘要

       在污水处理厂中，大部分病原体和抗生素抗性基因（ARGs）转移并集中在废活性污泥（WAS）中，这将造成严重的公共健康风险。在这项研究中，几种 WAS 预处理方法灭活大肠菌群/大肠杆菌的能力。大肠杆菌和 ARGs，以及随后大肠菌群/E 的再生。研究了处理污泥中的大肠杆菌和 ARGs/intI1。结果表明，具有连续羟基自由基生成的电芬顿 (EF) 可以有效地灭活大肠菌群/E。大肠杆菌在 60 分钟内（约 4 log 单位），然后是甲醇 (MT)、阳极氧化 (AO) 和酸化 (AT)。动力学分析表明失活主要发生在前 10 分钟。然而，所有研究的预处理方法对灭活 ARGs/intI1（<2 log 单位）的效率低于大肠菌群/E。大肠杆菌，而 EF 仍然具有最高的 ARGs/intI1 减少效率。机械超声处理 (ULS) 不能灭活大肠菌群/大肠杆菌。 WAS 中的大肠杆菌，但它可以有效地减少 ARGs/intI1。大肠菌群/大肠杆菌的高再生率。在 10 天内在处理过的 WAS 中观察到大肠杆菌，但在处理后孵育期间 ARGs/intI1 的丰度不断降低。我们的研究表明，EF 可以有效地消毒潜在的病原体，但是，WAS 中 ARGs/intI1 的减少需要进一步研究。

       In wastewater treatment plants, most of the pathogens and antibiotic resistant genes (ARGs) transferred into and concentrated in waste activated sludge (WAS), which would cause severe public health risks. In this study, the capabilities of several WAS pre-treatment approaches to inactivate coliforms/E. coli and ARGs, as well as the subsequent regrowth of coliforms/E. coli and ARGs/intI1 in treated sludge were investigated. The results showed that electro-Fenton (EF), with continuous hydroxyl radical generation, could efficiently inactivate coliforms/E. coli in 60 min (about 4 log units), followed by methanol (MT), anode oxidization (AO), and acidification (AT). Kinetic analysis showed that the inactivation mainly occurred in the first 10 min. However, the efficiencies of all studied pre-treatment approaches on inactivating ARGs/intI1 (<2 log units) were lower than coliforms/E. coli, whilst EF still had the highest efficiency of ARGs/intI1 reduction. Mechanical ultrasound treatment (ULS) could not inactivate coliforms/E. coli in WAS, but it could efficiently reduce ARGs/intI1. High regrowth rates of coliforms/E. coli were observed in the treated WAS in 10 days, but the abundances of ARGs/intI1 continuously reduced during the after-treatment incubation. Our study showed that EF could efficiently disinfect potential pathogens, however, the reduction of ARGs/intI1 in WAS need further investigation.

https://www.sciencedirect.com/science/article/abs/pii/S004896972036544X