摘要

       通过基因表达系列分析 (SAGE) 在激素反应性前列腺癌细胞系 LNCaP 中评估雄激素调节基因 (ARG) 的定量表达谱。代表 23,448 个已知基因或表达序列标签 (EST) 的 83,489 个 SAGE 标签和 1,655 个潜在的新序列揭示了 LNCaP 细胞的转录组，LNCaP 细胞是前列腺癌研究中最常用的细胞系。对照和 R1881 处理的 LNCaP 细胞之间转录的比较揭示了 136 个基因的诱导和 215 个基因对雄激素的抑制（p < 0.05）。引人注目的是，在我们的研究中发现的很大一部分（约 90%）ARGs 以前没有被描述为 ARGs。此处鉴定的 ARG 中有许多前列腺特异性转录因子。根据生化功能对 ARG 进行分类表明，在我们的实验系统中鉴定的绝大多数 ARG 似乎参与转录、剪接、核糖体生物发生、有丝分裂发生、生物能学和氧化还原过程的调节。雄激素信号传导的新方面之一包括参与 DNA 修复/重组过程的基因的雄激素调节。通过将我们的 LNCaP-C 和 LNCaP-T SAGE 文库与 NCBI-SAGE 网站上提供的 SAGE 标签文库进行比较，我们已经确定了 >200 种潜在的前列腺特异性/丰富的转录本。新的前列腺特异性基因和 ARG 的发现为确定这些基因在前列腺细胞生长、分化和肿瘤发生中的作用提供了独特的机会。 © 2001 Wiley-Liss, Inc.

       Quantitative expression profile of androgen-regulated genes (ARGs) was evaluated in the hormone-responsive prostate cancer cell line LNCaP by serial analysis of gene expression (SAGE). A total of 83,489 SAGE tags representing 23,448 known genes or expressed sequence tags (ESTs) and 1,655 potentially novel sequences have unraveled the transcriptome of LNCaP cells, the most common cell line used in prostate cancer research. Comparison of transcripts between control and R1881-treated LNCaP cells revealed the induction of 136 genes and repression of 215 genes in response to androgen (p < 0.05). Strikingly, a high fraction (∼90%) of ARGs identified in our study has not been described as ARGs previously. A number of prostate-specific transcription factors were among the ARGs identified here. Classification of the ARGs on the basis of biochemical functions revealed that a great majority of ARGs identified in our experimental system appear to be involved in regulation of transcription, splicing, ribosomal biogenesis, mitogenesis, bioenergetics and redox processes. One of the novel aspects of androgen signaling included androgen regulation of genes involved in DNA repair/recombination process. By comparing our LNCaP-C and LNCaP-T SAGE libraries with SAGE tag libraries available at the NCBI-SAGE website, we have identified >200 potential prostate specific/abundant transcripts. The discovery of new prostate-specific genes and ARGs provides a unique opportunity to determine the role of these genes in prostate cell growth, differentiation and tumorigenesis. © 2001 Wiley-Liss, Inc.

https://onlinelibrary.wiley.com/doi/full/10.1002/ijc.1196