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添加不同表面活性剂对鸡粪堆肥过程中抗生素抗性基因和intI1的影响

发布者:抗性基因网 时间:2021-07-05 浏览量:570

摘要

       好氧堆肥通常用于处理牲畜粪便。 在这项研究中,在堆肥过程中将生物表面活性剂(鼠李糖脂,RL)和化学表面活性剂(吐温 80,Tw)添加到鸡粪中,并确定了它们对 ARGs 和 intI1 变化的影响。 堆肥后,添加 Tw(1-4.7 个对数)的 ARGs 和 intI1 的 RA 减少通常大于添加 RL(0.8-3.7 个对数)和对照(CK)(0.3-2.6 个对数) ),并且 CK 的 ARGs 富集程度更高(0.9-1.8 log)。 ARG 曲线受温度和水溶性碳含量的显着影响。 RL 和 Tw 有效降低了生物可利用的 Cu 和 Zn 的浓度,从而阻碍了重金属对 ARG 的共同选择。 RL 和 Tw 对 ARGs 和 intI1 的影响表明,在堆肥后添加 Tw 的效果略高于 RL。

       Aerobic composting is usually employed to treat livestock manure. In this study, a bio-surfactant (rhamnolipid, RL) and chemical surfactant (Tween 80, Tw) were added to chicken manure during composting and their effects were determined on the variations in ARGs and intI1. After composting, the reductions in the RAs of ARGs and intI1 with the addition of Tw (1–4.7 logs) were generally greater than that with the addition of RL (0.8–3.7 logs) and the control (CK) (0.3–2.6 logs), and the enrichment of ARGs was higher with CK (0.9–1.8 logs). The ARG profiles were affected significantly by temperature and the water-soluble carbon contents. RL and Tw effectively reduced the concentrations of bio-available Cu and Zn, thereby hindering the co-selection of ARGs by heavy metals. The effects of RL and Tw on ARGs and intI1 indicate that the addition of Tw was slightly more effective than RL after composting.

https://www.sciencedirect.com/science/article/abs/pii/S0960852416309415