摘要

       包括大肠杆菌在内的细菌中抗菌素耐药基因 (ARG) 频率的增加可能对公共健康构成威胁。本研究调查了活性污泥和物理化学废水处理工艺对大肠杆菌分离株中 ARG 流行的影响。总共从两个活性污泥和两个物理化学市政处理厂的进水和出水（消毒前）中分离出 719 株大肠杆菌，并使用 DNA 微阵列进行基因分型。对于两种处理过程，大肠杆菌群体中 ARG 丰度的变化是不同的。活性污泥处理没有改变携带 ARG 的大肠杆菌的流行，但增加了大肠杆菌基因组中 ARG 的丰度，而物理化学处理降低了携带 ARG 的大肠杆菌的流行以及 ARG 在大肠杆菌基因组中的频率。大肠杆菌基因组。来自四个处理厂的大多数大肠杆菌分离物具有多种抗菌类别的 ARG，主要是氨基糖苷类、β-内酰胺类、喹诺酮类和四环素类。此外，这些分离株含有 DNA 插入序列元件，包括整合酶和转座酶。发现 ARG 的发生与毒力基因型之间存在显着的正相关。

       An increase in the frequency of antimicrobial resistance genes (ARGs) in bacteria including Escherichia coli could be a threat to public health. This study investigated the impact of activated sludge and physicochemical wastewater treatment processes on the prevalence of ARGs in E. coli isolates. In total, 719 E. coli were isolated from the influent and effluent (prior to disinfection) of two activated sludge and two physicochemical municipal treatment plants, and genotyped using DNA microarrays. Changes in the abundance of ARGs in the E. coli population were different for the two treatment processes. Activated sludge treatment did not change the prevalence of ARG-possessing E. coli but increased the abundance of ARGs in the E. coli genome while physicochemical treatment reduced both the prevalence of ARG-carrying E. coli as well as the frequency of ARGs in the E. coli genome. Most E. coli isolates from the four treatment plants possessed ARGs of multiple antimicrobial classes, mainly aminoglycoside, β-lactams, quinolone and tetracyclines. In addition these isolates harboured DNA insertion sequence elements including integrase and transposase. A significant positive association was found between the occurrence of ARGs and virulence genotypes.

https://www.sciencedirect.com/science/article/abs/pii/S0043135413009986