摘要
快速的人类活动和城市化加剧了抗生素抗性基因（ARG）诱发的人类健康风险。在这项研究中，使用高通量qPCR研究了中国东部条溪河13个流域的ARGs的分析。研究区ARGs高流行率表明抗生素耐药性污染严重（绝对丰度：6.1×108-2.1×1010拷贝/ L;相对丰度：0.033-0.158拷贝/细胞）。检测分析了常规水质（COD，TN，TP，NH3-N），细菌群落和移动基因元件（MGEs）作为ARGs迁移的影响因素。营养物质和MGEs与大多数ARGs呈正相关（P <0.05），细菌群落被认为是驱动ARGs改变的关键因素。随着土地利用研究和田间调查，农村地区，特别是耕地，有望成为ARGs转移和病原菌繁殖的高发区。与环境背景相比，在农村和城市地区观察到ARGs的传播和细菌群落的显着迁移，表明人类活动可能导致ARGs的传播。在这项研究中对ARGs的影响因素的分析可能揭示ARGs在城市河流中的维持和传播机制提供新的思路。
The rapid human activities and urbanization exacerbate the human health risks induced by antibiotic resistance genes (ARGs). In this study, the profiling of ARGs was investigated using high-throughput qPCR from water samples of 13 catchment areas in East Tiaoxi River, China. High prevalence of ARGs indicated significant antibiotic resistance pollution in the research area (absolute abundance: 6.1 × 108–2.1 × 1010 copies/L; relative abundance: 0.033–0.158 copies/cell). Conventional water qualities (COD, TN, TP, NH3-N), bacterial communities and mobile gene elements (MGEs) were detected and analyzed as factors of ARGs shift. Nutrient and MGEs showed positive correlation with most ARGs (P < 0.05) and bacteria community was identified as the key contributing factor driving ARGs alteration. With the land-use study and field investigation, country area, especially arable, was expected as a high spot for ARGs shift and pathogen breeding. Comparing to environmental background, promotion of ARGs and marked shift of bacterial community were observed in country and urban city areas, indicating that human activities may lead to the spread of ARGs. Analysis of factors affecting ARGs in this study may shed new light on the mechanism of the maintenance and propagation of ARGs in urban rivers.
https://www.sciencedirect.com/science/article/pii/S0269749117314379
