摘要

       研究了磁铁矿对猪粪厌氧消化 (AD) 过程中甲烷产生和抗生素抗性基因 (ARGs) 的影响。结果表明，甲烷产量最多增加了 16.1%，磁铁矿可以增强乙酰碎屑产甲烷作用，而不是功能基因定量和微生物群落分析所反映的氢营养产甲烷作用。丙酸盐降解率得到提高，并被同养氧化成 H+/e-/CO2 进行种间直接电子转移 (DIET) 和乙酸盐，其中 DIET 被磁铁矿进一步增强，乙酸盐通过同养乙酸盐氧化 (SAO) 转化为甲烷) 途径。磁铁矿主要影响 AD 中期的 ARGs，其中 ARGs 尤其是 ermF 显着富集。尽管 tetM 富集，mefA 最终减少，但磁铁矿最终并未影响总 ARGs 丰度。统计分析表明，磁铁矿主要通过微生物群落的变化影响 ARGs 的命运。 

       Effects of magnetite on methane production and fate of antibiotic resistance genes (ARGs) during anaerobic digestion (AD) of swine manure were investigated. Results showed that methane production was increased by maximum 16.1%, and magnetite could enhance the acetoclastic methanogenesis not hydrogenotrophic methanogenesis reflected by the functional gene quantification and microbial community analysis. The propionate degradation rate was improved, and it was syntrophic oxidized into H+/e−/CO2 for direct interspecies electron transfer (DIET) and acetate, where DIET was further enhanced by magnetite and the acetate was transformed into methane through syntrophic acetate oxidation (SAO) pathway. Magnetite mainly influenced the ARGs at the interim period of AD, where ARGs especially ermF were significantly enriched. Magnetite did not influence the total ARGs abundance at the end, although the tetM was enriched and mefA was reduced finally. Statistical analysis indicated that magnetite influenced the ARGs fate mainly through the changes of microbial community.

https://www.sciencedirect.com/science/article/abs/pii/S0960852419310776