摘要

       移动遗传元件 (MGE) 是抗生素抗性基因 (ARG) 跨环境传播的关键因素。在这里，我们使用宏基因组学来比较上游 (n = 6) 和下游 (n = 6) 收集的样本之间的河流抗性组（所有 ARG 的集合）和移动组（例如，整合酶、转座酶、整合子整合酶和插入序列公共区域“ISCR”元件） 6) 城市污水处理厂 (UWWTP)。与上游宏基因组相比，下游宏基因组的 ARG 丰度以及 MGE 标记物，尤其是整合子整合酶和 ISCR 元件的丰度显着增加。这些变化伴随着细菌的 16S rRNA 基因特征的普遍存在，这些细菌隶属于包括众所周知的人类和动物病原体的家族。我们的研究结果证实，处理过的废水的长期排放严重影响河流的抵抗力，不仅影响 ARG 的丰度和多样性，而且通过丰富各种 MGE 中的河流移动组来影响它们的潜在传播。

       Mobile genetic elements (MGEs) are key agents in the spread of antibiotic resistance genes (ARGs) across environments. Here we used metagenomics to compare the river resistome (collection of all ARGs) and mobilome (e.g., integrases, transposases, integron integrases and insertion sequence common region “ISCR” elements) between samples collected upstream (n = 6) and downstream (n = 6) of an urban wastewater treatment plant (UWWTP). In comparison to upstream metagenomes, downstream metagenomes showed a drastic increase in the abundance of ARGs, as well as markers of MGEs, particularly integron integrases and ISCR elements. These changes were accompanied by a concomitant prevalence of 16S rRNA gene signatures of bacteria affiliated to families encompassing well-known human and animal pathogens. Our results confirm that chronic discharges of treated wastewater severely impact the river resistome affecting not only the abundance and diversity of ARGs but also their potential spread by enriching the river mobilome in a wide variety of MGEs.

https://www.sciencedirect.com/science/article/abs/pii/S0269749117339349