摘要

      本研究调查了添加不同铜盐（硫酸铜、甘氨酸铜和这两种盐的 1:1 混合物）的猪粪嗜热 AD 过程中甲烷的产生和 ARG 的减少。结果表明，通过添加混合铜盐，甲烷产量增加了 28.78%。混合铜组有效地将总 ARGs 丰度降低了 26.94%，表明混合铜盐不会增加潜在的 ARGs 风险。混合铜盐对 AD 性能和 ARGs 去除的积极影响可能归因于低生物利用度。微生物群落分析表明，混合铜组中最高丰度的梭菌_MBA03 和甲烷杆菌可能导致甲烷产量增加。 Spearman 的秩相关分析阐明了由环境因素引起的微生物群落的演替是塑造 ARGs 谱的主要驱动因素。因此，混合铜盐可以替代动物饲料添加剂中的无机铜盐。

      This study investigated methane production and ARGs reduction during thermophilic AD of swine manure with the addition of different Cu salts (cupric sulfate, cupric glycinate, and the 1:1 mixture of these two salts). Results showed methane production was increased by 28.78% through adding mixed Cu salts. The mixed Cu group effectively reduced total ARGs abundance by 26.94%, suggesting mixed Cu salts did not promote the potential ARGs risk. The positive effects of mixed Cu salts on AD performance and ARGs removal might be ascribed to the low bioavailability. Microbial community analysis indicated the highest abundances of Clostridia_MBA03 and Methanobacterium in the mixed Cu group might cause the increased methane production. Spearman’s rank correlation analysis elucidated the succession in microbial community induced by environmental factors was the main driver for shaping ARGs profiles. Thus, mixed Cu salts could be an alternative to replace the inorganic Cu salt in animal feed additives.

https://www.sciencedirect.com/science/article/abs/pii/S0960852419319571