摘要

       20世纪80年代以来，中国东海沿岸地区经历了快速的城市化和工业化进程，造成了严重的环境污染。抗生素抗性基因（ARGs）被认为是一种具有潜在高风险的新兴污染物。从杭州湾（HB）、象山湾（XB）和台州湾（TB）采集沉积物样品，研究了27种ARGs和I类整合子-整合酶基因（intI1）在沿海地区的空间发生和分布。东海。 PCR结果显示，三个海湾的沉积物中经常存在11种ARGs和intI1。 qPCR结果进一步表明，磺胺类抗性是最普遍的ARG类型，抗生素靶点替代和保护是沉积物中最重要的抗性机制。关于 ARG 的亚型，sulI、tetW 和 dfrA13 是最丰富的 ARG，其中 sulI 在 TB 中较高（基于绝对和相对丰度），而 dfrA13 在 HB 中较高（基于相对丰度）。网络分析表明，intI1 与 tetC、sulI、sulII 和 blaPSE-1 具有显着相关性。石油是关键的连接因素，与 sulI、sulII 和 blaPSE-1 呈正相关。此外，重金属与营养物质和有机污染物的共同作用可能对沿海沉积物中 ARGs 的归宿至关重要。

       The coastal area of the East China Sea has experienced rapid urbanization and industrialization in China since 1980s, resulting in severe pollution of its environments. Antibiotic resistance genes (ARGs) are regarded as a kind of emerging pollutant with potential high risk. The sediment samples were collected from Hangzhou Bay (HB), Xiangshan Bay (XB), and Taizhou Bay (TB) to investigate the spatial occurrence and distribution of 27 ARGs and class I integron–integrase gene (intI1) in the coastal area of the East China Sea. The PCR results showed the frequent presence of 11 ARGs and intI1 in the sediments of the three bays. The qPCR results further showed that sulfonamide resistance was the most prevalent ARG type and antibiotic target replacement and protection were the most important resistance mechanisms in the sediments. Regarding the subtype of ARGs, sulI, tetW, and dfrA13 were the most abundant ARGs, in which sulI was higher in TB (based on both the absolute and relative abundances) and dfrA13 was higher in HB (based on the relative abundances). The network analysis revealed that intI1 had significant correlations with tetC, sulI, sulII, and blaPSE-1. Oil was the key connected factor, which had positive connections with sulI, sulII, and blaPSE-1. In addition, the joint effect of heavy metals and nutrients & organic pollutants might be crucial for the fate of ARGs in the coastal sediments.

https://www.sciencedirect.com/science/article/abs/pii/S100107421832895X