摘要

       研究了抗生素抗性基因（ARGs）和重金属抗性基因（HMRGs）对两种不同温度序列（即嗜温-嗜热和嗜热-嗜温）的反应。通过嗜温-嗜热序列比通过嗜热-嗜温序列实现了更高的总 ARG（二十一种靶向亚型）和 HMRG（三种靶向亚型）的去除。嗜温-嗜热的序列显示对总 ARG 的去除率最高，但嗜热-嗜温的序列证明更适合去除 1 类整合子 (intI1)。相关性分析表明，intI1 与 tetG、tetQ、tetX、sul2、aac(6')-lb-cr、blaTEM、ermB 和 floR 显着相关。高通量测序显示，与嗜热-嗜温序列相比，嗜温-嗜热序列 TPAD 去除了更多的人类细菌病原体 (HBP)。此外，在 ARGs 和 HBPs 之间观察到显着的正相关。例如，肺炎支原体与包括 tetE、tetQ、tetX、tetZ、sul1、sul2、aac(6')-lb-cr 和 floR 在内的几种 ARG 呈显着正相关。

       Response of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs) to two different temperature sequences (i.e., mesophilic-thermophilic and thermophilic-mesophilic) were investigated. Higher removal of total ARGs (twenty-one targeted subtypes) and HMRGs (three targeted subtypes) was achieved by the mesophilic-thermophilic sequence than by the thermophilic-mesophilic sequence. The sequence of mesophilic-thermophilic showed the highest removal of total ARGs, but the sequence of thermophilic-mesophilic proved more suitable for removal of class 1 integrons (intI1). Correlation analysis indicated that intI1 correlated significantly with tetG, tetQ, tetX, sul2, aac(6′)-lb-cr, blaTEM, ermB and floR. High-throughput sequencing revealed that the mesophilic-thermophilic sequence TPAD removed more human bacterial pathogens (HBPs) than did the thermophilic-mesophilic sequence. Also, significantly positive correlation was observed between ARGs and HBPs. For instance, Mycoplasma pneumonia showed significantly positive correlation with several ARGs including tetE, tetQ, tetX, tetZ, sul1, sul2, aac(6′)-lb-cr and floR.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418309489