摘要

       近年来，各种抗生素的引入有助于有效降低细菌感染的影响。然而，某些细菌中抗生素抗性的进化和获得导致死亡率增加。这是抗生素滥用和过度处方的结果。尽管与感染性细菌的抗生素耐药性相关的困难，但关于耐药基因的分布、多样性和起源的信息量有限。抗生素药物发现和抗菌素耐药性的公共卫生监测领域已经开始利用基因组和宏基因组学测序的能力。高通量基因组工具的应用为环境健康监测提供了新的途径。这包括基于宏基因组学的抗生素耐药性决定因素研究 宏基因组学有助于提高对自然和临床环境中抗生素耐药性机制的理解。本综述讨论了通过宏基因组学进行抗生素耐药性分析领域的最新发现和未来障碍。

       In recent times, the introduction of a varied range of antibiotics has helped to effectively reduce the effects of bacterial infections. However, the evolution and procurement of antibiotic-resistance in certain bacteria has led to increased cases of mortality. This is a result of abuse and overprescription of antibiotics. Despite the difficulties associated with antibiotic resistance in infectious bacteria, only a limited amount of information is known regarding the distribution, diversity, and origin of resistance genes. The area of antibiotic drug discovery and public health monitoring of antimicrobial resistance have started to utilize the capabilities of genomic and metagenomics sequencing. The application of high-throughput genomic tools provides new avenues for environmental health monitoring. This includes metagenomics-based investigation of antibiotic resistance determinants Metagenomics has helped to improve the understanding of the mechanisms of antibiotic resistance in natural and clinical environments. This review discusses recent findings and future obstacles in the area of antibiotic resistance analysis through metagenomics.

https://www.sciencedirect.com/science/article/pii/B9780128188828000139