摘要

       本研究旨在量化采用不同深度处理系统 [生物曝气过滤器、人工湿地和紫外线 (UV) 消毒] 的三个城市污水处理厂 (WWTP) 中抗生素抗性基因 (ARG) 的浓度和去除情况。在废水和生物固体样品中检测了 tetM、tetO、tetQ、tetW、sulI、sulII、intI1 和 16S rDNA 基因的浓度。在市政污水处理厂中，观察到 ARG 减少了 1-3 个数量级，并且在三个不同处理工艺的市政污水处理厂之间没有发现差异（p > 0.05）。在高级处理系统中，人工湿地的 ARGs 减少了 1-3 个数量级，曝气生物过滤器中的 ARGs 减少了 0.6-1.2 个数量级，但没有观察到紫外线消毒的明显减少。发现人工湿地和生物过滤器之间存在显着差异（p < 0.05），人工湿地和紫外线消毒之间存在显着差异（p < 0.05）。在人工湿地中，在去除 ARG 和 16S rDNA 基因方面观察到显着相关性（R2 = 0.391–0.866；p < 0.05）。人工湿地不仅具有与污水处理厂相当的ARG去除值（p > 0.05），而且在ARG相对丰度去除方面具有优势，应优先考虑成为污水处理厂进一步衰减ARG的深度处理系统。

       This study aimed at quantifying the concentration and removal of antibiotic resistance genes (ARGs) in three municipal wastewater treatment plants (WWTPs) employing different advanced treatment systems [biological aerated filter, constructed wetland, and ultraviolet (UV) disinfection]. The concentrations of tetM, tetO, tetQ, tetW, sulI, sulII, intI1, and 16S rDNA genes were examined in wastewater and biosolid samples. In municipal WWTPs, ARG reductions of 1–3 orders of magnitude were observed, and no difference was found among the three municipal WWTPs with different treatment processes (p > 0.05). In advanced treatment systems, 1–3 orders of magnitude of reductions in ARGs were observed in constructed wetlands, 0.6–1.2 orders of magnitude of reductions in ARGs were observed in the biological aerated filter, but no apparent decrease by UV disinfection was observed. A significant difference was found between constructed wetlands and biological filter (p < 0.05) and between constructed wetlands and UV disinfection (p < 0.05). In the constructed wetlands, significant correlations were observed in the removal of ARGs and 16S rDNA genes (R2 = 0.391–0.866; p < 0.05). Constructed wetlands not only have the comparable ARG removal values with WWTP (p > 0.05) but also have the advantage in ARG relative abundance removal, and it should be given priority to be an advanced treatment system for further ARG attenuation from WWTP.

https://pubs.acs.org/doi/abs/10.1021/es401091y