摘要

       本研究调查了添加三种浓度（0、80 和 160mg/L）的纳米零价铁 (nZVI) 对甲烷产量和厌氧消化 (AD) 过程中抗生素抗性基因 (ARG) 命运的影响。 牛粪。 nZVI的添加有效地提高了甲烷产率，80mg/L nZVI显着提高了6.56%，160mg/L nZVI显着提高了6.43%。 AD 后添加 160mg/L nZVI 加速了 ARG 和 Tn916/1545 丰度的降低。 微生物群落分析表明，nZVI 主要增加了具有水解和酸化作用的细菌的丰度，而它降低了不动杆菌的丰度。 冗余分析表明，移动遗传元件的变化对ARGs的命运贡献最大。 结果表明，160mg/L nZVI 是降低 AD 中 ARGs 风险的合适添加剂。

       This study investigated the effects of adding nano-zerovalent iron (nZVI) at three concentrations (0, 80, and 160 mg/L) on the methane yield and the fate of antibiotic resistance genes (ARGs) during the anaerobic digestion (AD) of cattle manure. The addition of nZVI effectively enhanced the methane yield, where it significantly increased by 6.56% with 80 mg/L nZVI and by 6.43% with 160 mg/L nZVI. The reductions in the abundances of ARGs and Tn916/1545 were accelerated by adding 160 mg/L nZVI after AD. Microbial community analysis showed that nZVI mainly increased the abundances of bacteria with roles in hydrolysis and acidogenesis, whereas it reduced the abundance of Acinetobacter. Redundancy analysis indicated that the changes in mobile genetic elements made the greatest contribution to the fate of ARGs. The results suggest that 160 mg/L nZVI is a suitable additive for reducing the risks due to ARGs in AD.

https://www.sciencedirect.com/science/article/abs/pii/S0960852419309186