摘要

       本研究的目的是调查澳大利亚韦里比河流域灌溉用水中抗生素抗性基因 (ARG) 的发生情况，包括河水和再生污水（再生水）。使用 PCR 检测分析法筛选一年内收集的再生水样品中是否存在 ARG。再生水样品中 ARG 的存在与同一时期从 Werribee 河流域采集的水样进行了对比，这些水样来自为不同程度的城市和农业影响选择的五个点。采集的54个河水样品中，分别有2个（4%）、2个（4%）、0个和0个对甲氧西林、磺胺类、庆大霉素和万古霉素耐药基因呈阳性，而11个再生水样中有6个对甲氧西林呈阳性(9%) 和磺胺 (45%)。 ARG 的存在/不存在似乎与其他测量的水质参数无关。河水中 ARGs 的检出率低表明，尽管河流质量很差，但尚未受到 ARGs 的严重污染。再生水中 ARGs 的普遍存在表明，未来需要对这一重要的农业水源进行监测。

       The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the reclaimed water samples were contrasted with that of water samples taken from the Werribee River Basin, collected over the same time period, from five points selected for varying levels of urban and agricultural impact. Of the 54 river water samples collected, 2 (4%), 2 (4%), 0 and 0 were positive for methicillin, sulfonamide, gentamicin and vancomycin-resistant genes, respectively, while 6 of 11 reclaimed water samples were positive for methicillin (9%) and sulfonamide (45%). The presence/absence of ARGs did not appear to correlate with other measured water quality parameters. The low detection of ARGs in river water indicates that, regardless of its poor quality, the river has not yet been severely contaminated with ARGs. The greater prevalence of ARGs in reclaimed water indicates that this important agricultural water source will need to be monitored into the future.

https://iwaponline.com/jwh/article/8/3/521/18131/Occurrence-of-antibiotic-resistance-genes-in