摘要

       抗生素抗性细菌 (ARB) 和抗生素抗性基因 (ARG) 是导致严重全球健康问题的新兴污染物。尽管紫外线（UV）照射等消毒可以去除部分 ARB 和 ARGs，但污水处理厂的出水中仍然存在残留的 ARB 和 ARGs。
共轭转移是 ARGs 风险的主要关注点，关于紫外线消毒对进入环境的流出物中未灭活 ARB 转移能力的影响知之甚少。因此，在实验室条件下研究了紫外线照射和再激活对 ARB 共轭转移能力的影响，重点研究了紫外线照射后的存活细菌和重新激活的细菌及其后代。实验结果表明，即使是 1mJ/cm2 的紫外线消毒也能显着降低存活细菌的结合转移频率。然而，由紫外线诱导的有活力但不可培养的状态细胞可以通过光活化和暗修复来重新激活，并保持与未处理菌株相同水平的转移能力。这一发现对于重新考虑紫外线照射出水的后安全性和微生物安全控制策略是必不可少的。

       Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are the emerging contaminants leading to a serious worldwide health problem. Although disinfection like ultraviolet (UV) irradiation could remove part of ARB and ARGs, there still are residual ARB and ARGs in the effluent of wastewater treatment plants.
Conjugative transfer is main concern of the risk of ARGs and little is known about the effects of UV disinfection on the transfer ability of the non-inactivated ARB in the effluent which will enter the environment. Hence the influences of UV irradiation and reactivation on ARB conjugative transfer ability were studied under laboratory condition, focusing on the survival bacteria from UV irradiation and the reactivated bacteria, as well as their descendants. The experimental results imply that even 1 mJ/cm2 UV disinfection can significantly decrease the conjugative transfer frequency of the survival bacteria. However, viable but not culturable state cells induced by UV can reactivate through both photoreactivation and dark repair and retain the same level of transfer ability as the untreated strains. This finding is essential for re-considering about the post safety of UV irradiated effluent and microbial safety control strategies were required.

https://www.sciencedirect.com/science/article/abs/pii/S0045653519304369