摘要

       了解在堆肥过程中影响抗生素抗性基因 (ARG) 传播的主要驱动因素对于去除 ARG 很重要。在这项研究中，将三种水平的泰乐菌素（25、50 和 75mgkg-1，以干重计）添加到猪粪和用棉秆堆肥的对照中。每个处理重复三次，并用不同水平的泰乐菌素测定 ARG 谱。根据堆肥时间将前 35 个属和 ARGs 配置文件聚类在一起。组合堆肥参数（温度、pH、NH4+-N、NO3-N 和水分含量）占潜在宿主细菌变化总变化的 78.4%。此外，根据冗余分析，选定的五个堆肥参数和六个门（包括 25 个潜在宿主细菌属）分别解释了 ARG 谱中 46.9% 和 30.7% 的变异。堆肥过程中 ARGs 的变化主要受潜在宿主细菌的动力学而不是整合子的动力学以及 bio-Cu 和 bio-Zn 的选择压力的影响。

       Understanding the main drivers that affect the spread of antibiotic resistance genes (ARGs) during the composting process is important for the removal of ARGs. In this study, three levels of tylosin (25, 50, and 75 mg kg–1 on a dry weight basis) were added to swine manure plus a control, which was composted with cotton stalks. Each treatment was repeated in triplicate and the ARG profiles were determined with different levels of tylosin. The top 35 genera and ARGs profiles were clustered together based on the composting time. Combined composting parameters (temperature, pH, NH4+-N, NO3-N, and moisture content) accounted for 78.4% of the total variation in the changes in the potential host bacteria. In addition, the selected five composting parameters and six phyla (including 25 potential host bacterial genera) explained 46.9% and 30.7% of the variation in the ARG profiles according to redundancy analysis, respectively. The variations in ARGs during the composting process were mainly affected by the dynamics of potential host bacteria rather than integrons and the selective pressure due to bio-Cu and bio-Zn.

https://www.sciencedirect.com/science/article/abs/pii/S0304389418304928