摘要

       环境抗菌素耐药性（AMR）因其对人类健康的巨大风险而引起越来越多的关注。 本研究的目的是调查从中国对虾海水养殖环境中回收的副溶血性弧菌分离株（n=114）的 AMR 和基因分型。 分离株对链霉素（78.9%）、氨苄青霉素（64.9%）和庆大霉素（53.5%）的耐药率很高。 此外，多重耐药性非常普遍（61.4%），其中 95.9% 的这些氨苄青霉素耐药菌株主要由 blaCARB-17 介导。 令人惊讶的是，多西环素、氟苯尼考和甲氧苄啶/磺胺甲恶唑 (TMP/SMZ) 抗性基因出现在易感分离株中。 此外，114 个分离株被分组为独特的脉冲场凝胶电泳模式。 这些发现表明需要在海水养殖场谨慎使用抗微生物剂，以控制抗药性副溶血性弧菌的传播。 

       Environmental antimicrobial resistance (AMR) has drawn increasing attention due to its great risk to human health. The aim of this study was to investigate AMR and genotyping of Vibrio parahaemolyticus isolates (n = 114) recovered from shrimp mariculture environment in China. The isolates exhibited a high rate of resistance to streptomycin (78.9%), ampicillin (64.9%) and gentamicin (53.5%). Furthermore, multi-drug resistance was highly prevalent (61.4%), in which 95.9% of these ampicillin-resistant isolates were primarily mediated by blaCARB-17. Surprisingly, doxycylcine, florfenicol, and trimethoprim/sulfamethoxazole (TMP/SMZ) resistance genes occurred in susceptible isolates. Moreover, 114 isolates were grouped into unique pulsed field gel electrophoresis patterns. These findings suggest the need for the prudent use of antimicrobial agents on mariculture farms, in order to control the dissemination of antimicrobial resistant V. parahaemolyticus.

https://www.sciencedirect.com/science/article/abs/pii/S0025326X18306568