摘要

       研究了代表性抗生素抗性基因 (ARG) 在高温和中温条件下对实验室规模的两相（产酸/产甲烷相）厌氧消化过程的响应。相关的微生物群落和细菌病原体通过 16S rRNA 基因测序进行表征。两相高温消化减少了 tetA、tetG、tetX、sul1、ermB、dfrA1、dfrA12 和 intI1 的存在，表现出 0.1–0.72 log 单位去除；相比之下，tetO、tetW、sul3、ermF 和 blaTEM 甚至相对于饲料增加，而 sul2 没有显着减少。嗜热消化的产酸阶段主要负责减少这些基因的数量，而随后的产甲烷阶段导致它们数量的反弹。相比之下，除 ermB 和 blaTEM 外，两相中温消化过程不会导致所有 ARG 和 intI1 的数量减少。在两阶段厌氧消化过程中，ARGs 模式与变形菌和放线菌相关。

       The response of representative antibiotic resistance genes (ARGs) to lab-scale two-phase (acidogenic/methanogenic phase) anaerobic digestion processes under thermophilic and mesophilic conditions was explored. The associated microbial communities and bacterial pathogens were characterized by 16S rRNA gene sequencing. A two-phase thermophilic digestion reduced the presence of tetA, tetG, tetX, sul1, ermB, dfrA1, dfrA12 and intI1 exhibiting 0.1–0.72 log unit removal; in contrast, tetO, tetW, sul3, ermF and blaTEM even increased relative to the feed, and sul2 showed no significant decrease. The acidogenic phase of thermophilic digestion was primarily responsible for reducing the quantity of these genes, while the subsequent methanogenic phase caused a rebound in their quantity. In contrast, a two-phase mesophilic digestion process did not result in reducing the quantity of all ARGs and intI1 except for ermB and blaTEM. ARGs patterns were correlated with Proteobacteria and Actinobacteria during the two-phase anaerobic digestion.

https://www.sciencedirect.com/science/article/abs/pii/S0960852416303807