摘要

       源自污水污泥的抗生素抗性基因 (ARG) 的传播被强调为一个突出的健康威胁。本研究建立了一种使用一步启动策略的嗜热厌氧消化器，以快速去除污水污泥中的四环素和磺胺类抗性基因。在从中温到高温条件的启动期间至少节省了 20 天。根据 16S rDNA 扩增子测序结果和预测的宏基因组方法，成功启动很大程度上依赖于核心嗜热微生物种群（例如厚壁菌门、变形菌门、放线菌）的快速定植。用于底物降解和甲烷产生的微生物代谢基因途径也通过一步模式增加。此外，实时定量 PCR 方法表明，大多数靶向四环素和磺胺类药物抗性基因 ARG（sulI、tetA、tetO、tetX）在高温消化过程中被大量去除（去除效率 > 80%）。网络分析表明，ARGs的消除归因于它们在高温下水平（intI1项目）和垂直（潜在宿主）转移相关元素的下降。该研究表明，废水固体的快速启动嗜热厌氧消化将是减少各种 ARG 数量的合适技术。

       Spread of antibiotic resistance genes (ARGs) originating from sewage sludge is highlighted as an eminent health threat. This study established a thermophilic anaerobic digester using one-step startup strategy to quickly remove tetracycline and sulfonamides resistance genes from sewage sludge. At least 20 days were saved in the startup period from mesophilic to thermophilic condition. Based on the results of 16S rDNA amplicons sequencing and predicted metagenomic method, the successful startup largely relied on the fast colonization of core thermophilic microbial population (e.g. Firmicutes, Proteobacteria, Actinobacteria). Microbial metabolic gene pathways for substrate degradation and methane production was also increased by one-step mode. In addition, real-time quantitative PCR approach revealed that most targeted tetracycline and sulfonamides resistance genes ARGs (sulI, tetA, tetO, tetX) were substantially removed during thermophilic digestion (removal efficiency > 80%). Network analysis showed that the elimination of ARGs was attributed to the decline of their horizontal (intI1 item) and vertical (potential hosts) transfer-related elements under high-temperature. This research demonstrated that rapid startup thermophilic anaerobic digestion of wastewater solids would be a suitable technology for reducing quantities of various ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0048969717323173