摘要

       目的：我们研究了婴儿肠道抗性组的几种抗生素抗性基因 (ARG) 在出生后头几个月的纵向发展。 材料和方法：分析了 120 名 5、13 和 31 周龄婴儿的粪便样本，并进行 qPCR 以检测几种 ARG。 结果： ermB、tetM 和 tetQ 的 ARGs 患病率显着增加，而 aac(6')-aph(2') 的 ARGs 患病率下降。 出生方式和母乳喂养显着影响了 tetQ 的流行。 与细菌分类群的相关性表明，某些 ARG 的波动（部分）归因于拟杆菌定植率的变化。 结论：出生后不久肠道微生物群中 ARG 的获得发生，并且抵抗组的组成在几个月的过程中波动，反映了微生物群落结构的变化。

       Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth. Materials & methods: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs. Results: The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6′)-aph(2′). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates. Conclusion: Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.

https://www.futuremedicine.com/doi/abs/10.2217/fmb.15.154