摘要

       抗生素抗性基因在河流系统中的传播是一个新兴的环境问题，因为它们对水生生态系统和公共健康构成潜在威胁。在这项研究中，我们使用液滴数字聚合酶链反应 (ddPCR) 来评估中国渭河干流沿线 13 个监测点的临床相关抗生素抗性基因 (ARG) 的污染。使用 ddPCR 分析了六个临床相关的 ARG 和一个 I 类整合子整合酶 (intI1) 基因，并使用 MiSeq 测序基于细菌 16S rRNA V3-V4 区域评估细菌群落。结果表明，渭河水样中以变形菌门、放线菌门、蓝藻门和拟杆菌门为主。与相对低丰度的 strA、mecA 和 vanA 基因（0-1.94 拷贝/mL）相比，在地表水样品中检测到更高丰度的 blaTEM、strB、aadA 和 intI1 基因（103 至 105 拷贝/mL）。基于网络分析，八个细菌属被确定为 intI1 基因和三个 ARG（strA、strB 和 aadA）的可能宿主。结果表明细菌群落结构和水平基因转移与ARGs的变化有关。查看全文

       The spread of antibiotic resistance genes in river systems is an emerging environmental issue due to their potential threat to aquatic ecosystems and public health. In this study, we used droplet digital polymerase chain reaction (ddPCR) to evaluate pollution with clinically relevant antibiotic resistance genes (ARGs) at 13 monitoring sites along the main stream of the Weihe River in China. Six clinically relevant ARGs and a class I integron-integrase (intI1) gene were analyzed using ddPCR, and the bacterial community was evaluated based on the bacterial 16S rRNA V3–V4 regions using MiSeq sequencing. The results indicated Proteobacteria, Actinobacteria, Cyanobacteria, and Bacteroidetes as the dominant phyla in the water samples from the Weihe River. Higher abundances of blaTEM, strB, aadA, and intI1 genes (103 to 105 copies/mL) were detected in the surface water samples compared with the relatively low abundances of strA, mecA, and vanA genes (0–1.94 copies/mL). Eight bacterial genera were identified as possible hosts of the intI1 gene and three ARGs (strA, strB, and aadA) based on network analysis. The results suggested that the bacterial community structure and horizontal gene transfer were associated with the variations in ARGs. View Full-Text

https://www.mdpi.com/1660-4601/15/4/708