摘要

从废水中回收的磷铁矿有望将磷作为肥料回收到土壤中。然而，鸟粪石的应用可能会促使土壤和植物中抗生素抗性的增加。本研究使用定量PCR和克隆文库分析，研究了鸟粪石施用和生物炭修正对根际土壤和叶鞘中整合子丰度和基因盒背景的影响。微观实验表明，1类整合子在所有样品中都是最普遍的，根际的浓度和相对丰度均高于叶绿素。大部分抗性基因盒与编码对氨基糖苷类，β-内酰胺类和氯霉素的抗性基因相关。 Struvite应用显着增加了抗生素抗性基因盒在根际和叶面的遗传多样性。然而，生物炭修改减弱了phyllosphere应用于phyllosphere的1类整合子抗生素抗性基因盒库的增加效应。这些发现强调了人类活动是整合子基因盒库的来源，并提出使用生物炭修正作为减轻环境中抗生素抗性的替代手段的可能性。

Struvite recovered from wastewater is promising for recycling phosphorus into soil as fertilizers. However, struvite application may prompt the proliferation of antibiotic resistance in soil and plant. This study examined the impacts of struvite application and biochar amendment on integrons abundance and gene cassette contexts in rhizosphere soil and phyllosphere using quantitative PCR and clone library analysis. Microcosm experiments revealed that class 1 integron was the most prevalent in all samples, with higher concentration and higher relative abundance in rhizosphere than those in phyllosphere. The majority of resistancegene cassettes were associated with genes encoding resistance to aminoglycosides, beta-lactams and chloramphenicols. Struvite application significantly increased the genetic diversity of antibiotic resistance gene cassettes in both rhizosphere and phyllosphere. However, biochar amendment attenuated the increasing effect of struvite application exerting on the class 1 integron antibiotic resistance gene cassette pool in phyllosphere. These findings highlighted human activities to be the source of integron gene cassette pool and raised the possibility of using biochar amendment as an alternative mean for mitigatingantibiotic resistance in environments.

https://www.ncbi.nlm.nih.gov/pubmed/29539595