摘要

使用传统的污泥处理工艺难以消除存在于污水污泥中的多种抗生素抗性基因（ARG）。迄今为止，关于污泥蚯蚓堆肥过程中ARG的命运，还鲜为人知。本研究旨在探讨蚯蚓对四环素和氟喹诺酮类抗性基因的命运的影响，以及通过对比蚯蚓和不蚯蚓两种污泥稳定系统对污泥进行蚯蚓堆肥期间的整合子的影响。与没有蚯蚓的对照相比，蚯蚓混合显着降低了四环素和氟喹诺酮类抗性基因和int1的丰度，并且parC完全去除。 ARGs的变化与环境因素，水平基因转移，细菌群落组成和蚯蚓在蚯蚓堆肥期间有关。此外，蚯蚓强烈影响编码ARG和Int1的可能的宿主细菌，从而减少蚯蚓堆产品中的致病菌。这些结果意味着蚯蚓堆肥可以有效减少污泥中的四环素和氟喹诺酮抗性基因。

Diverse antibiotic resistance genes (ARGs) present in sewage sludge are difficult to be eliminated using conventional sludge treatment processes. To date, little remains known on the fate of the ARGs during vermicomposting of sludge. This study aimed to investigate the effect of earthworms on the fate of tetracycline and fluoroquinolone resistance genes, and integrons during vermicomposting of sewage sludge through contrasting two systems of sludge stabilization with and without earthworms. Compared to the control without earthworms, vermicomposting significantly (p < 0.05) decreased the abundances of tetracycline and fluoroquinolone resistance genes and int1, with complete removal for parC. Variations in ARGs were associated with environmental factors, horizontal gene transfer, bacterial community composition, and earthworms during vermicomposting. In addition, earthworms strongly affected the possible host bacteria encoding ARGs and Int1, abating the pathogenic bacteria in vermicomposting product. These results imply that vermicomposting could effectively reduce tetracycline and fluoroquinoloneresistance genes in the sludge.