摘要
      抗生素耐药性基因的传播增加了对人类健康和环境安全的风险。本工作研究了通过化学调理和随后的中温厌氧消化（MAD）处理废物活性污泥（WAS）过程中ARGs丰度和细菌群落进化的控制。亚铁活化氧代和过氧化氢（PMS-Fe2+和H2O2-Fe2+）以及热活化氧代的不同化学氧化过程(PMS@80℃），并对氯化铁（FeCl3）和灭活的氧代酮（PMS）进行了比较。PMS@80温度使大多数ARGs的绝对丰度降低了10.6–99.3%，使总ARGs降低了66.3%。有趣的是，氧化预处理使大多数ARG的相对丰度增加而不是降低。MAD与PMS@80℃预处理使总ARGs的绝对丰度增加了51.6%，而其他MAD工艺使其减少了8.6-47.4%。PMS-Fe2+和PMS@80温度对MAD中甲烷生成的抑制作用从98.3降至81.7，对VSS的抑制作用为94.4mL/g。在基于PMS的预处理后，MAD出水显示出高丰度的Arcobacter属，范围为8.1-17.4%，可能与硫氧化、硝酸盐还原和blaVEB富集有关。由于污泥中具有极高的竞争性有机物，以自由基为导向的化学氧化很难改善MAD对ARGs的去除。
Abstract
The dissemination of antibiotic resistance genes (ARGs) increases risks towards human health and environmental safety. This work investigates the control of ARGs abundance and bacterial community evolution involved in waste activated sludge (WAS) treatment by chemical conditioning and subsequent mesophilic anaerobic digestion (MAD). The different chemical oxidation processes of ferrous iron-activated oxone and hydrogen peroxide (PMS-Fe2+ and H2O2-Fe2+) and thermal-activated oxone (PMS@80 ℃) were investigated, and the ferric chloride (FeCl3) and inactivated oxone (PMS) were compared. PMS@80 ℃ decreased the absolute abundance of most ARGs by 10.6–99.3% and that of total ARGs by 66.3%. Interestingly, oxidation pretreatment increased rather than decreased the relative abundance of most ARGs. MAD with PMS@80 ℃ pretreatment increased the absolute abundance of total ARGs by 51.6%, and other MAD processes decreased it by 8.6–47.4%. PMS-Fe2+ and PMS@80 ℃ negatively inhibited methane production from 98.3 to 81.7 and 94.4 mL/g VSS in MAD. MAD effluent showed high abundance of Arcobacter genus in the range of 8.1–17.4% upon PMS-based pretreatment, possibly related to sulfur oxidation, nitrate reduction, and blaVEB enrichment. The radicals-orientated chemical oxidation can hardly improve the ARGs elimination by MAD due to the extremely high competitive organics in sludge.

https://www.sciencedirect.com/science/article/abs/pii/S0304389421029708