摘要
      粪肥好氧堆肥中的抗生素抗性基因是一种潜在的环境污染物。因此，减少ARGs的丰度至关重要。研究了添加三维印刷填充剂（3DBA）对猪粪好氧堆肥中ARGs的影响。与对照组相比，不同添加剂量的3DBA可最大限度地减少总ARGs 5.98%，四环素抗性基因14.02%，大环内酯抗性基因9.65%，磺酰胺抗性基因20.59%，研究发现，氧浓度对ARGs的减少至关重要，具有规则孔隙率和均匀尺寸的3DBA通过改变耗氧量间接调节宿主微生物的活性和MGEs的丰度，最终降低ARGs垂直或水平转移的风险。总的来说，添加3DBA是减少好氧堆肥中ARGs丰度的有效策略。
Abstract
Antibiotic resistance genes (ARGs) in manure aerobic composting are a potential environmental pollutant. Therefore, reducing the abundance of ARGs is crucial. The effects of adding three-dimensional printed bulking agents (3DBAs) on ARGs in aerobic composting of swine manure were investigated in this study. Compared with the control group, 3DBAs with different addition dosages can greatest reduce the total ARGs by 5.98%, tetracycline resistance genes by 14.02%, macrolide resistance genes by 9.65%, and sulfonamide resistance genes by 20.59%. By further combining physicochemical parameters, host microorganisms, and mobile genetic elements (MGEs) for analysis, it was found that oxygen concentration was vital for ARGs reduction, and 3DBAs with regular porosity and uniform size indirectly regulate the activity of host microorganisms and MGEs abundance by changing the oxygen consumption, finally reducing vertical or horizontal ARGs transfer risks. Overall, 3DBAs addition is an effective strategy to reduce the abundance of ARGs in aerobic composting.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422008185