摘要
      养牛场废水作为抗生素抗性基因（ARGs）的重要部分，受到了广泛关注。在废水处理过程中检测到细胞内和细胞外ARG（iARGs和eARGs），包括固液分离、厌氧调节、上流式厌氧污泥床（UASB）消化、缺氧-缺氧-缺氧（A2O2）工艺、膜生物反应器（MBR）和臭氧消毒。根据宏基因组测序，选择10个丰富的ARGs作为靶ARGs。由于UASB、A2O2、MBR和臭氧消毒可以逐渐降低大多数ARG的浓度，因此原废水中的总目标iARGs和eARGs的浓度分别为6.12×107和3.24×106拷贝数/mL，最终出水中的总靶iARGs浓度分别为3.79×103和3.95×105拷贝数/mL。ARGs的浓度与几乎所有的废水质量指标都呈正相关。iARGs与拟杆菌门、厚壁菌门和螺旋体门之间也呈正相关，表明这三个门中的细菌可能是ARGs的主要宿主。废水质量指标和细菌群落组成影响了牛废水处理过程中ARGs的分布和去除。
Abstract
Cattle farm wastewater, as a significant reservoir for antibiotic resistance genes (ARGs), has received wide attention. Intracellular and extracellular ARGs (iARGs and eARGs) were detected during wastewater treatment, including solid–liquid separation, anaerobic regulation, upflow anaerobic sludge blanket (UASB) digestion, an anoxic–oxic-anoxic–oxic (A2O2) process, a membrane bioreactor (MBR), and ozone disinfection. Ten abundant ARGs were chosen as the target ARGs according to metagenomic sequencing. The concentrations of the total target iARGs and eARGs were 6.12 × 107 and 3.24 × 106 copy numbers/mL in raw wastewater, and then 3.79 × 103 and 3.95 × 105 copy numbers/mL in final effluent, because UASB, A2O2, MBR and ozone disinfection can gradually reduce the concentrations of most ARGs. The concentrations of ARGs were positively correlated with almost all wastewater quality indicators. Positive correlation was also observed between iARGs and Bacteroidetes, Firmicutes and Spirochaetes, indicating that the bacteria in these three phyla might be the main hosts of ARGs. Wastewater quality indicators and bacterial community composition affected the distribution and removal of ARGs during cattle wastewater treatment.

https://www.sciencedirect.com/science/article/abs/pii/S096085242101614X