摘要
目的
研究和比较同居狗和主人粪便中的抗微生物基因。
方法和结果
使用高通量qPCR从35只狗和35位主人的粪便样本中筛选出34种临床相关ARG。总共，狗和主人组中分别存在24个和25个不同的ARG。这些家庭平均有9.9个ARG，狗和主人平均共享3.3个ARG。有6岁以上狗的家庭（3.5，四分位间距2.75-5.0）中ARGs的共享量显著高于有年幼狗的家庭中（2.5，四分位数间距2.0-3.0）（p=0.02）。狗比主人拥有更多的mecA和氨基糖苷类抗性基因。
结论
狗和主人可以作为多种ARG的宿主，这些ARG属于几种抗微生物耐药性类别。在狗和主人身上同时存在一定比例的相同耐药性基因，这表明在缺乏抗菌药物选择的情况下，狗和人类肠道之间的ARG传播是一个次要问题。
研究的意义和影响
这项研究深入了解了常见的狗和人类肠道耐药性，有助于改善狗和人之间ARG传播的风险评估知识库。
Abstract
Aims
To investigate and compare antimicrobial resistance genes (ARGs) in faeces from cohabiting dogs and owners.

Methods and Results
DNA from faecal samples from 35 dogs and 35 owners was screened for the presence of 34 clinically relevant ARGs using high throughput qPCR. In total, 24 and 25 different ARGs were present in the dog and owner groups, respectively. The households had a mean of 9.9 ARGs present, with dogs and owners sharing on average 3.3 ARGs. ARGs were shared significantly more in households with dogs over 6 years old (3.5, interquartile range 2.75–5.0) than in households with younger dogs (2.5, interquartile range 2.0–3.0) (p = 0.02). Dogs possessed significantly more mecA and aminoglycoside resistance genes than owners.

Conclusions
Dogs and owners can act as reservoirs for a broad range of ARGs belonging to several antimicrobial resistance classes. A modest proportion of the same resistance genes were present in both dogs and owners simultaneously, indicating that ARG transmission between the dog and human gut is of minor concern in the absence of antimicrobial selection.

Significance and Impact of the Study
This study provides insight into the common dog and human gut resistomes, contributing to an improved knowledge base in risk assessments regarding ARG transmission between dogs and humans.

https://academic.oup.com/jambio/article/133/2/1027/6989222?login=false