摘要
      供水水库是重要的饮用水资源之一。它们的水质直接影响人类健康。然而，水库沉积物在抗生素抗性基因（ARGs）传播方面没有得到足够的关注，尽管它们反映了供水水库的长期ARGs污染。此外，供水水库沉积物中的物理化学参数通常优于其他介质中的物理物理参数。因此，媒体的主要ARGs生物驱动因素将展示其独特的特征。本研究采集了中国中部10个供水水库的沉积物样本，并用宏基因组方法测定了抗生素耐药性。结果显示，在水库沉积物中检测到174种ARG（18种ARG类型）。此外，多药、磺酰胺和万古霉素ARGs是沉积物样品中的主要ARGs。抗大环内酯类微囊藻在水库沉积物中普遍存在（检测频率为100%，平均百分比为0.35%），对人类健康构成潜在风险。此外，Mantel试验和VPA的结果表明，流动遗传元素（MGE）是水库沉积物ARG含量的更重要的生物驱动因素，而不是细菌群落。
Abstract
Water supply reservoirs form one of the critical drinking water resources. Their water quality directly affects human health. However, reservoir sediments have not received adequate attention in antibiotic resistance genes (ARGs) dissemination, though they reflect long-term ARGs contamination of water supply reservoirs. Moreover, the physicochemical parameters in water supply reservoir sediments are generally better than those in the other media. Thus, the main ARGs biotic drivers of the media would demonstrate their unique characteristics. In this study, sediment samples were collected from 10 water supply reservoirs in central China, and the antibiotic resistomes were determined with the metagenomic method. As revealed from the results, 174 ARGs (18 ARG types) were detected in the reservoir sediment. Besides, multidrug-, sulfonamide-, and vancomycin-ARGs were the dominant ARGs in the sediment samples. The macrolide-resistant Microcystis was prevalent (100% detection frequency with 0.35% average percentage) in reservoir sediments and posed potential risks to human health. Furthermore, the results of the Mantel test and VPA demonstrated that mobile genetic elements (MGEs) were the more essential biotic drivers in ARG contents of reservoir sediments rather than the bacteria community.

https://link.springer.com/article/10.1007/s11356-021-18095-w