摘要
      抽象图像
多种营养群落栖息在土壤动物肠道中，包括细菌、真菌和原生生物，它们被认为是抗生素耐药性基因（ARGs）的隐藏库。然而，很少有研究关注ARGs与土壤动物肠道中多营养群落之间的关系。在这里，我们研究了多营养群落对土壤木虱肠道ARGs变化的贡献。研究结果显示，木虱肠道中存在多种丰富的ARG。网络分析进一步表明，关键生态模块成员和ARGs之间有着密切的联系，这表明关键生态集群中的多营养群落可能在木虱肠道中ARGs的变化中发挥着关键作用。此外，污水污泥的长期应用显著改变了木虱肠道抗性和界间群落。变异分配分析表明，长期施用污水污泥后，木虱肠道中真菌群落对ARGs变异的贡献大于细菌和原生生物群落。总之，我们的研究结果表明，与农业实践（如污泥施用）相关的肠道微生物群的变化可以在很大程度上改变生态相关土壤动物的肠道界间网络，并对抗生素耐药性产生影响，这促进了我们对陆地生态系统中ARGs的微生态驱动因素的理解。
Abstract
Abstract Image
Multitrophic communities inhabit in soil faunal gut, including bacteria, fungi, and protists, which have been considered a hidden reservoir for antibiotic resistance genes (ARGs). However, there is a dearth of research focusing on the relationships between ARGs and multitrophic communities in the gut of soil faunas. Here, we studied the contribution of multitrophic communities to variations of ARGs in the soil woodlouse gut. The results revealed diverse and abundant ARGs in the woodlouse gut. Network analysis further exhibited strong connections between key ecological module members and ARGs, suggesting that multitrophic communities in the keystone ecological cluster may play a pivotal role in the variation of ARGs in the woodlouse gut. Moreover, long-term application of sewage sludge significantly altered the woodlice gut resistome and interkingdom communities. The variation portioning analysis indicated that the fungal community has a greater contribution to variations of ARGs than bacterial and protistan communities in the woodlice gut after long-term application of sewage sludge. Together, our results showed that changes in gut microbiota associated with agricultural practices (e.g., sewage sludge application) can largely alter the gut interkingdom network in ecologically relevant soil animals, with implications for antibiotic resistance, which advances our understanding of the microecological drivers of ARGs in terrestrial ecosystem.

https://pubs.acs.org/doi/abs/10.1021/acs.est.2c02471