摘要
      利用具有人工湿地的微生物燃料电池（MFC CWs）来消除抗生素最近引起了广泛的关注。然而，必须提高MFC CWs中的抗生素去除效率，并且抗生素抗性基因（ARGs）的积累仍然是一个难以控制的问题。本研究试图通过分别和/或同时在MFC CWs的阳极和阴极中添加海绵铁（s-Fe0）和过氧化钙来提高合成废水中抗生素的去除率并减少ARGs。结果表明，在MFC CWs中加入s-Fe0和过氧化钙可以使磺胺甲恶唑（SMX）和四环素（TC）的去除率分别提高0.8–1.3%和6.0–8.7%。其中，s-Fe0还显著降低了总sul和tet基因的84.10–94.11%和49.61–60.63%。此外，s-Fe0提高了电压输出、功率密度、库仑效率，并降低了电抗器的内阻。电极层的强化对微生物群落的组成和功能产生了显著影响，这促使MFC CWs中抗生素去除、ARGs积累和生物电生成的转变。考虑到MFC CWs的整体性能，在MFC CWs阳极区域添加s-Fe0被发现是去除抗生素和减少ARGs积累的有效策略。
Abstract
Using microbial fuel cells with constructed wetlands (MFC-CWs) for eliminating antibiotics has recently attracted extensive attention. However, antibiotic removal efficiencies in MFC-CWs must be enhanced, and the accumulation of antibiotic resistant genes (ARGs) remains an unmanageable issue. This study tries to enhance the antibiotic removal in synthetic wastewater and reduce ARGs by adding sponge iron (s-Fe0) and calcium peroxide to the anode and cathode of MFC-CWs, respectively, and/or simultaneously. The results demonstrated that adding s-Fe0 and calcium peroxide to MFC-CWs could improve the removal efficiencies of sulfamethoxazole (SMX) and tetracycline (TC) by 0.8–1.3% and 6.0–8.7%. Therein, s-Fe0 also significantly reduced 84.10–94.11% and 49.61–60.63% of total sul and tet genes, respectively. Furthermore, s-Fe0 improved the voltage output, power density, columbic efficiency, and reduced the internal resistance of reactors. The intensification to the electrode layers posed a significant effect on the microbial community composition and functions, which motivated the shift of antibiotic removal, accumulation of ARGs and bioelectricity generation in MFC-CWs. Given the overall performance of MFC-CWs, adding s-Fe0 to the anode region of MFC-CWs was found to be an effective strategy for removing antibiotics and reducing the accumulation of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0048969721052979