摘要
抽象图像
抗生素耐药性基因（ARGs）作为一种新兴的空气污染物，越来越受到公众和环境健康的关注。准确监测和定量空气中的低剂量ARGs对于为人口健康管理提供循证公共风险评估至关重要。通过这项工作，我们引入了一种便携式和灵敏的电化学（EC）生物分析平台，用于检测空气中的ARGs，该平台依赖于表面启动的ARGs-杂交的EC电流响应。这些用于EC ARGs传感的低成本芯片表现出良好的热稳定性（25～60°C）和长期稳定性（6天）。生物传感器系统的原型表现出超低的检测极限（7.4fM）和优异的抗干扰性能。首次成功应用EC传感器从真实世界的PM2.5（空气动力学直径小于2.5μm的颗粒物）样本中识别和定量两种类型的ARG（floR和bla-TEM基因）。获得了瑞士伯尔尼（城市地区）、巴塞尔（郊区）、苏黎世（城市区域）、锡昂（机场公路区域）和里吉（农村和高海拔地区）PM2.5的采集结果，与凝胶电泳分析结果一致。结果表明，我们的EC生物传感器为机载ARG检测提供了一种可靠且有吸引力的替代方法。
Abstract
Abstract Image
Antibiotic resistance genes (ARGs) as an emerging airborne pollutants are a significant and growing concern for public and environmental health. Accurate surveillance and quantitation of low-dose ARGs in the air have become critical in providing evidence-based public risk assessment for population health management. With this work, we introduced a portable and sensitive electrochemical (EC) bioanalytical platform for detecting airborne ARGs, which relied on the EC current response of surface-initiated ARGs hybridization. These low-cost chips for EC ARGs sensing exhibited good thermal (25∼60 °C) and long-term stability (6 days). The prototype of the biosensor system demonstrated an ultralow limit of detection (7.4 fM) and excellent anti-interference performance. For the first time, an EC sensor was applied successfully to identify and quantitate two types of ARGs (floR and bla-TEM genes) from real-world PM2.5 (particulate matter with aerodynamic diameters less than 2.5 μm) samples. Results for PM2.5 collected from Bern (urban area), Basel (suburban area), Zürich (urban area), Sion (airport highway area), and Rigi (rural and high-altitude area) in Switzerland were obtained, in favorable agreement with gel electrophoresis analysis. The results indicated that our EC biosensor offered a reliable and attractive alternative to the current methods for airborne ARG detection.

https://pubs.acs.org/doi/abs/10.1021/acsestengg.2c00063