摘要
      抗微生物耐药性（AMR）的出现是对公众健康的全球性威胁。抗菌药物用于动物生产和人类医学，有助于抗生素耐药性基因（ARGs）在环境中的循环。野生动物可能是病原体和耐药细菌的宿主。此外，人为压力会影响它们的抵抗力。这项工作旨在研究欧洲最重要的猎物之一马鹿粪便微生物组的AMR。为此，采用高通量qPCR方法筛选大量ARGs，并测定指示菌的抗菌敏感性。鉴定出了几种对不同种类抗生素产生耐药性的基因，其中最丰富的是四环素ARGs。其他基因也被认为是当前和未来对人类健康的威胁，其中一些基因相对丰富。尽管抗生素耐药性总体水平较低，但仍分离出耐多药大肠杆菌和肠球菌。这些结果强调了迫切需要了解AMR在野生动物中的起源和传播。因此，考虑到“一个健康”的概念，像这样的研究表明，有必要制定监测计划来防止耐药菌株和ARGs的传播。
Abstract
The emergence of antimicrobial resistance (AMR) is a global threat to public health. Antimicrobials are used in animal production and human medicine, which contribute to the circulation of antibiotic resistance genes (ARGs) in the environment. Wildlife can be reservoirs of pathogens and resistant bacteria. Furthermore, anthropogenic pressure can influence their resistome. This work aimed to study the AMR of the faecal microbiome of red deer, one of the most important game species in Europe. To this end, a high-throughput qPCR approach was employed to screen a high number of ARGs and the antimicrobial susceptibility of indicator bacteria was determined. Several genes that confer resistance to different classes of antibiotics were identified, with the most abundant being tetracycline ARGs. Other genes were also present that are considered current and future threats to human health, and some of these were relatively abundant. Multidrug-resistant E. coli and Enterococcus spp. were isolated, although the overall level of antibiotic resistance was low. These results highlight the pressing need to know the origin and transmission of AMR in wildlife. Thus, and considering the One Health concept, studies such as this one shows the need for surveillance programs to prevent the spread of drug-resistant strains and ARGs.

https://www.sciencedirect.com/science/article/pii/S0048969722009238