摘要
      在国内剩余污泥的蚯蚓堆肥过程中，温度对抗生素抗性基因（ARGs）的影响仍知之甚少。在三种不同的温度（15°C、20°C和25°C）下对剩余污泥进行了蚯蚓复合实验，以研究ARGs、细菌群落的命运及其在该过程中的关系。相对于15°C和20°C的情况，25°C的蚯蚓堆肥并没有显著减弱目标ARG。在15°C和20°C的蚯蚓堆肥过程中，qnrA、qnrS和tetM基因的动力学遵循一级动力学模型。温度显著影响最终产品的细菌多样性，在25°C时香农指数最低。该属（气单胞菌属和几丁质菌科）在25°C下的存在可能有助于基因（qnrA、qnrS和tetM）的反弹。研究表明，20°C是一个合适的蚯蚓堆肥温度，可以同时达到ARGs的最高去除效率和最终产品的良好生物稳定性。
Abstract
Effect of temperature on antibiotic resistance genes (ARGs) during vermicomposting of domestic excess sludge remains poorly understood. Vermicomposting experiment with excess sludge was conducted at three different temperatures (15 °C, 20 °C, and 25 °C) to investigate the fate of ARGs, bacterial community and their relationship in the process. The vermicomposting at 25 °C did not significantly attenuate the targeted ARGs relative to that at 15 °C and 20 °C. The dynamics of qnrA, qnrS, and tetM genes during vermicomposting at 15 °C and 20 °C followed the first-order kinetic model. Temperature remarkably impacted bacterial diversity of the final products with the lowest Shannon index at 25 °C. The presence of the genus (Aeromonas and Chitinophagaceae) at 25 °C may contribute to the rebound of the genes (qnrA, qnrS and tetM). The study indicates that 20 °C is a suitable vermicomposting temperature to simultaneously reach the highest removal efficiency of the ARGs and the good biostability of the final product.

https://www.sciencedirect.com/science/article/abs/pii/S0013935121019551