摘要
      宠物粪便（如猫和狗的粪便）中的ARG特征及其对环境安全的潜在威胁尚不清楚。在这项研究中，在45只猫和28只狗的粪便样本中检测到了ARGs，并进行了扩散实验，以评估ARGs扩散到空气中的风险。结果表明，猫和狗粪便中ARGs的丰度较高，狗粪便中的ARGs丰度（0.89±0.17拷贝/细菌细胞）显著高于猫粪便中的（0.46±0.09拷贝/细胞）（P<0.05），是影响ARG谱变异的主要因素，分别占猫粪便和狗粪便变异的31.6%和32.4%。理化因素（尤其是NH4+-N）和年龄也通过影响细菌群落间接影响ARG谱的变化。此外，猫粪便和狗粪便中的ARGs扩散到空气中，但没有证据表明这种扩散对环境安全和人类健康构成威胁。这些结果可为健康动物饲养和ARG污染的防治提供参考数据。
Abstract
The ARG profiles in pet feces, such as cat and dog feces, and their potential threat to environmental safety are still unclear. In this study, ARGs in 45 cat and 28 dog fecal samples were detected, and a diffusion experiment was performed to assess the risk of ARGs diffusion into the air. The results showed that the abundances of ARGs in cat feces and dog feces were high, and the abundance in dog feces (0.89 ± 0.17 copies/bacterial cell) was significantly higher than that in cat feces (0.46 ± 0.09 copies/bacterial cell) (P < 0.05). The bacterial community, especially Firmicutes and Desulfobacterota in cat feces, and Proteobacteria in dog feces, was the main factor affecting the variation in the ARG profiles, contributing to 31.6% and 32.4% of the variation in cat feces and dog feces, respectively. Physicochemical factors (especially NH4+-N) and age also indirectly affected the variation in the ARG profiles by affecting the bacterial community. In addition, the ARGs in cat feces and dog feces diffused into the air, but there was no evidence that this diffusion posed a threat to environmental safety and human health. These results can provide reference data for healthy animal breeding and the prevention and control of ARG pollution.

https://www.sciencedirect.com/science/article/abs/pii/S0269749121019461