摘要
      在本研究中，在20–55°C的厌氧消化（AD）中研究了抗生素抗性基因（ARGs）、移动基因元件（intI1）和人类细菌病原体（HBPs）对添加粪肥生物炭（1–10 g/L）处理奶牛粪便的反应。通过定量PCR分析了12个ARGs，包括5个四环素抗性基因、2个磺酰胺抗性基因、两个大环内酯抗性基因、3个β-内酰胺抗生素抗性基因和intI1。高通量测序数据与推定538个HBP的数据库相匹配。在所有样品中观察到ARGs（tetO和ermB除外）和intI1的显著去除。粪肥生物炭显著去除了ARGs和HBPs；然而，在某些条件下也观察到了负面影响。这是第一项通过向AD中添加粪肥生物炭来探索ARGs和HBPs命运的研究。
Abstract
In this study, the response of antibiotic resistance genes (ARGs), mobile gene elements (intI1), and human bacterial pathogens (HBPs) to addition of manure biochar (1–10 g/L) was studied in anaerobic digestion (AD) at 20–55 °C for treating dairy manure. Twelve ARGs comprising five tetracycline resistance genes, two sulfonamide resistance genes, two macrolide resistance genes, three β-lactam antibiotic resistance genes, and intI1 were analyzed by quantitative PCR. High-throughput sequencing data were matched against a database of putative 538 HBPs. Significant removal of ARGs (except for tetO and ermB) and intI1 was observed in all the samples. Manure biochar resulted in significant removal of ARGs and HBPs; however, negative effects were also observed in some conditions. This is the first study to provide to explore the fates of ARGs and HBPs by adding manure biochar to AD.

https://www.sciencedirect.com/science/article/abs/pii/S004565352201671X