摘要
      抗生素抗性基因（ARGs）被认为是一种新的环境污染物，通过厌氧发酵去除猪粪中的ARGs是一个至关重要的课题。本研究探讨了猪粪厌氧发酵过程中初始pH值（3、5、7、11）对细胞内和细胞外ARGs（iARGs和eARGs）以及可移动遗传元件（MGE）的影响。结果发现，发酵过程中的初始pH为酸性（pH 3和5），这有助于去除6个eARG和7个iARG。类似地，细胞内和细胞外MGE在初始pH为3和5时被有效消除。MGE和四个ARG的丰度在初始pH为7和11时富集。酸性条件可以极大地降低微生物群落的多样性和丰度，从而去除MEG和ARGs。这些发现对于ARG的风险评估和管理至关重要。
Abstract
Antibiotic resistance genes (ARGs) are considered to be a new environmental pollutant and the removal of ARGs from swine manure by anaerobic fermentation was a crucial topic. This research discusses effects of initial pH values (3, 5, 7, 11) on intracellular and extracellular ARGs (iARGs and eARGs) as well as mobile genetic elements (MGEs) during anaerobic fermentation of swine manure had been examined. The initial pH during fermentation was found to be acidic (pH 3 and 5) in results, which was conducive to the removal of six eARGs and seven iARGs. Similarly, intracellular and extracellular MGEs were effectively eliminated with an initial pH of 3 and 5. The abundance of MGEs and four ARGs were enriched with an initial pH of 7 and 11. Acidic conditions can greatly deduce the diversity as well as abundance of the microbial community, ensuing removal of MEGs and ARGs. These findings are critical for risk assessment and management of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422005934