摘要
      本研究旨在通过分析细菌多样性、代谢多样性和抗生素抗性基因（ARGs），研究不同来源的处理废水灌溉后对土壤的影响。与先前报道的废水中的ARGs进行了比较分析，以了解相对于对照土壤方案，ARGs从处理过的废水到灌溉土壤的流动性。不动杆菌属、伯克霍尔德菌属和假单胞菌属是所有样本中数量最多的属。所有样本的代谢基因丰度表明，它们对天然矿物循环利用做出了突出贡献。观察到的大多数ARGs编码对克林霉素、卡那霉素A、大环内酯类、巴龙霉素、大观霉素和四环素的耐药性。除机构处理点（M）外，在大多数情况下，经处理的污水回用似乎不会提高土壤中的ARG水平，在那里，氨基糖苷类、β-内酰胺类和磺酰胺类的ARG大量存在于经处理的废水和灌溉的土壤中。本研究发现了来自不同来源的废水处理的重要性以及处理后的废水在灌溉中的再利用影响。这项研究还强调了更好地理解ARGs从水到土壤的流动性。
Abstract
Present study aims to investigate how is soil affected following irrigation with treated effluents of different origins by analysing the bacterial diversity, metabolic diversity and antibiotic resistance genes (ARGs). Comparative analysis with previously reported ARGs in effluents was performed to understand the mobility of ARGs from treated wastewater to the irrigated soil with respect to the control soil regimen. Acinetobacter, Burkholderia and Pseudomonas were observed as the most abundant genera in all the samples. The metabolic gene abundance of all the samples suggests a prominent contribution to natural mineral recycling. Most abundant ARGs observed encode resistance for clindamycin, kanamycin A, macrolides, paromomycin, spectinomycin and tetracycline. Treated effluent reuse did not appear to enhance the ARG levels in soils in most cases except for institutional treatment site (M), where the ARGs for aminoglycosides, β-lactams and sulfonamides were found to be abundantly present in both treated effluent and the irrigated soil. This study finds the importance of wastewater treatment from different origins and the impact of treated wastewater reuse in irrigation. This study also emphasises on the better understanding of ARGs mobility from water to soil.

https://www.sciencedirect.com/science/article/abs/pii/S0045653522026686