摘要
      近年来，抗生素抗性基因（ARGs）的命运已在各种环境介质中得到揭示。也就是说，抵抗粘菌素和碳青霉烯类的基因的出现引起了广泛的关注。然而，尽管黄河是中国第二长的河流，但人们对其ARGs和污染源的污染状况仍知之甚少。本研究确定了黄河河南段的ARG污染水平，并评估了水产养殖业在ARG传播中的作用。结果表明，在黄河沉积物中共检测到9种ARG，黄河中ARG的总含量在7.27至245.45 RPKM之间。Sul1和sul2是占主导地位的ARGs，磺胺类药物的大量使用、水平基因转移和广泛的细菌宿主导致了这两个基因的流行。Spearman相关分析结果表明，养殖业对黄河ARGs的影响很小。网络分析表明，机会病原体假单胞菌是sul1、tetG和ANT（3′′）-IIa的潜在宿主，可能对人类健康构成风险。
Abstract
The fate of antibiotic resistance genes (ARGs) has been revealed in various environmental media in recent years. Namely, the emergence of genes that resist colistin and carbapenems has attracted wide attention. However, the pollution condition of ARGs and sources in the Yellow River is still little understood, despite the river being the second longest in China. The present study determined the levels of ARG pollution in the Henan section of the Yellow River and evaluated the role of the aquaculture industry in the spread of ARGs. As revealed by the results, a total of 9 types of ARGs were detected in the sediments of the Yellow River, and the total ARG content in the Yellow River ranges from 7.27 to 245.45 RPKM. Sul1 and sul2 are the dominant ARGs, and the huge usage of sulfonamides, horizontal gene transfer, and wide bacteria host contribute to the prevalence of these two genes. The results of Spearman correlation analysis indicate that the breeding industry has little influence on ARGs in the Yellow River. Network analysis reveals that the opportunistic pathogen Pseudomonas is the potential host of sul1, tetG, and ANT(3′′)-IIa, which can pose a risk to human health.

https://www.mdpi.com/1660-4601/19/16/10420