摘要
      在韩国，首次通过量化抗生素抗性基因（ARGs）并分析16个流通式养殖场在鱼类饲养时间之前（BF）和之后（AF）30min的进水（in）和出水中的物理化学参数，研究了鱼类饲料对抗生素抗性基因丰度变化的空间和时间影响。AF样品中ARGs的绝对丰度是BF样品的5倍，是in样品的12倍。投喂鱼类后，出水中的氨、总氮、悬浮物和浊度等物理化学参数值分别显著增加了21.6倍、4.2倍、2.6倍和1.65倍。从空间上看，与附近全罗南道的养殖场（3.4×10−5–8.3×10−3）相比，济州岛的养殖场表现出更高的ARGs相对丰度（3.02×10−4–6.1×10−2）。在季节上，夏季和秋季的样本显示出比冬季和春季更高的ARGs丰度。为了评估食物链和公共卫生的风险，有必要进行进一步的研究，以探索这些ARGs的致病潜力。
Abstract
For the first time, both spatial and temporal effects of fish feed on changes in abundance of antibiotic resistance genes (ARGs) were investigated in South Korea via quantifying ARGs and analyzing physicochemical parameters in the influent (IN) and effluent before (BF) and 30 min after (AF) the fish feeding time of sixteen flow-through fish farms. The absolute abundance of ARGs in AF samples was 5 times higher than in BF and 12 times higher than in IN samples. Values of physicochemical parameters such as ammonia, total nitrogen, suspended solids and turbidity in the effluent significantly increased by 21.6, 4.2, 2.6 and 1.65 times, respectively, after fish feeding. Spatially, the fish farms on Jeju Island exhibited higher relative abundance (3.02 × 10−4 – 6.1 × 10−2) of ARGs compared to the farms in nearby Jeollanam-do (3.4 × 10−5 – 8.3 × 10−3). Seasonally, samples in summer and autumn showed a higher abundance of ARGs than in winter and spring. To assess risk to the food chain as well as public health, further studies are warranted to explore the pathogenic potential of these ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0013935122005047