摘要
      为了有效处理泰乐菌素菌丝渣（TMDs），采用综合元组学（基因组学、元基因组学和代谢组学）和qPCR方法研究了TMDs发酵过程中泰乐菌肽的快速去除机制和ARGs的动力学。结果表明，在第7天，超过86%的泰乐菌素被降解，而与填充剂的类型无关。泰乐菌素的快速去除主要归因于糖单孢菌的去mycarose反应（GH3）和酯酶水解（C7MYQ7），以及芽孢杆菌的过氧化氢酶-过氧化物酶氧化（A0A077JB13）。此外，水分含量和可移动遗传元素对控制ARGs的反弹至关重要。以玉米芯为填充剂的发酵处理TC21对抗生素抗性细菌（链霉菌、假单胞菌、norank_f__Sphingobacteriaceae和Paenalcaligenes）和Intl1的去除率（98.8%）显著高于其他三种处理（88.3%），这对于有效地减小电阻是至关重要的。
Abstract
For efficient treatment of tylosin mycelial dregs (TMDs), rapid tylosin removal mechanism and dynamics of ARGs during TMDs fermentation were investigated using integrated meta-omics (genomics, metaproteomics and metabolomics) and qPCR approaches. The results showed that over 86% of tylosin was degraded on day 7 regardless of the type of bulking agents. The rapid removal of tylosin was mainly attributed to de-mycarose reaction (GH3) and esterase hydrolysis (C7MYQ7) of Saccharomonospora, and catalase-peroxidase oxidation of Bacillus (A0A077JB13). In addition, the moisture content and mobile genetic elements were vital to control the rebound of ARGs. The removal efficiency of antibiotic resistant bacteria (Streptomyces, Pseudomonas, norank_f__Sphingobacteriaceae, and Paenalcaligenes) and Intl1 (98.8%) in fermentation treatment TC21 with corncob as the bulking agent was significantly higher than that in other three treatments (88.3%). Thus, appropriate bulking agents could constrain the abundance of antibiotic resistant bacteria and Intl1, which is crucial to effectively reduce the resistance.

https://www.sciencedirect.com/science/article/abs/pii/S096085242200339X