摘要
      抗生素耐药性基因（ARGs）可能导致细菌耐药性，使用抗生素会促进ARGs的传播。水产养殖中使用了大量的抗生素，但很少关注鱼类肠道中的抗生素耐药性。在这项研究中，在中国北方的一个城市采集了九种中国淡水和海鱼，以测试它们肠道内容物中抗生素和ARGs的残留量。结果表明，从肠道内容物中检测到4种抗生素，包括多西环素（DOX）、四环素（TC）、磺胺甲恶唑（SMX）和罗红霉素（ROX），其中检测量最大的抗生素是沙丁胺磷中的ROX（2.83 μg kg−1）。从肠道内容物中检测到10种ARGs，包括strA、strB、ermB、blaTEM、oxa-30、qnrB、qnrD、sul1、sul2和tetB，以及一种整合素intI1。最丰富的ARGs是blaTEM。相关分析显示，淡水鱼和海水鱼之间存在巨大差异。研究结果可以提高我们对食用鱼中抗生素和ARGs残留的认识。
Abstract
Antibiotic resistance genes (ARGs) may lead to bacterial resistance and using antibiotics will promote ARGs spread. Large amounts of antibiotics were used in aquaculture, but little attention was paid to the antibiotic resistant in fish gut. In this study, nine kinds of Chinese freshwater and marine fish were acquired in a city of northern China to test the amount of antibiotics and ARGs residues in their intestinal contents. The results showed that 4 kinds of antibiotics were detected from the intestinal contents, including Doxycycline (DOX), Tetracycline (TC), Sulfamethoxazole (SMX) and Roxithromycin (ROX), and the antibiotics with the largest detected amount was ROX in Sardinops sagax (2.83 μg kg−1). Ten kinds of ARGs were detected from the intestinal contents, including strA, strB, ermB, blaTEM, oxa-30, qnrB, qnrD, sul1, sul2 and tetB, as well as one type of integron intI1. The most abundant ARGs were blaTEM. Correlation analysis showed huge difference between freshwater fish and marine fish. The results can improve our understanding of the antibiotics and ARGs residues in edible fish.

https://www.tandfonline.com/doi/abs/10.1080/03601234.2022.2051401