摘要
      本研究研究了氯霉素（CAP）压力对微生物燃料电池（MFC）系统和普通厌氧反应器中反硝化性能、发电性能、抗生素抗性基因（ARGs）表达和微生物群落结构的影响。结果表明，MFC在CAP胁迫下具有较好的脱氮和降解CAP的性能。浓度为10 mg L–1的CAP显著抑制了两个系统中的反硝化作用。然而，在20 mg L–1 CAP下，由于同时携带ARGs和反硝化基因的红环藻科电子转移优势细菌、假单胞菌反硝化细菌和伯克霍尔德菌科的丰度增加，反硝化能力恢复，似乎没有受到影响，尤其是在MFC系统中。此外，qPCR结果表明，MFC可以促进抗性基因的水平转移，使MFC在CAP压力下表现优异。高通量测序结果表明，在不同CAP浓度下，细菌群落发生了显著变化，尤其是优势细菌，如地杆菌、盘诺杆菌、假单胞菌、红环菌科和伯克霍尔德菌科。总之，本研究表明MFC是一种更有前途的方法，并为MFC处理实际废水提供了理论指导。
Abstract
This study investigated the effects of chloramphenicol (CAP) pressure on denitrification performance, electricity generation performance, the expression of antibiotic resistance genes (ARGs), and microbial community structure in microbial fuel cell (MFC) system and plain anaerobic reactor. The results showed that the MFC had better denitrification and CAP degradation performances under CAP stress. CAP at a concentration of 10 mg L–1 significantly inhibited denitrification in both systems. However, at 20 mg L–1 CAP, the denitrification capacity recovered and seemed unaffected, especially in MFC system, due to the increased abundance of Rhodocyclaceae electron transfer dominant bacteria, pseudomonas denitrifying bacteria, and Burkholderiaceae carrying both ARGs and denitrifying genes. In addition, the qPCR results showed that the MFC could promote the horizontal transfer of resistance genes, making MFC superior under CAP pressure. The high-throughput sequencing results showed that bacterial communities significantly changed under different CAP concentrations, especially the dominant bacteria, such as Geobacter, Pannonibacter, Pseudomonas, Rhodocyclaceae and Burkholderiaceae. In conclusion, this study reveals MFC is a more promising method and provides theoretical guidance for the treatment of practical wastewater by MFCs.

https://www.sciencedirect.com/science/article/abs/pii/S1369703X22000985