摘要

生物炭在厌氧消化过程中对氮的保护有积极作用，但其对抗生素抗性基因（ARG）的影响尚不清楚。 因此，研究了生物质炭（0,5,20和50g / L）对牛粪废水厌氧消化过程中环境风险的影响。 结果表明，5g / L生物炭降低了5/13 ARG的相对丰度（RA），而20g / L生物炭显着降低了消化产物中ARG的总RA，其中ISCR1的RA比 控制。 生物炭主要通过影响厚壁菌和变形菌的RAs影响ARGs的分布，20g / L生物炭的影响大于5g / L。 移动遗传因素也影响ARG概况，尤其是intI2和ISCR1。 牛养殖场废水厌氧消化系统中添加20g / L生物炭可降低ARG的环境风险。

Biochar has positive effects on nitrogen conservation during anaerobic digestion, but its impacts on antibiotic resistance genes (ARGs) are unclear. Therefore, the effect of biochar (0, 5, 20, and 50 g/L) on the environmental risk of ARGs during cattle manure wastewater anaerobic digestion were investigated. The results showed that 5 g/L biochar reduced the relative abundances (RAs) of 5/13 ARGs while 20 g/L biochar significantly reduced the total RAs of ARGs in the digestion products, where the RA of ISCR1 was 0.89 log lower than the control. Biochar mainly affected the distribution of ARGs by influencing the RAs of Firmicutes and Proteobacteria, and the influence of 20 g/L biochar was greater than that of 5 g/L. Mobile genetic elements also influenced the ARG profiles, especially intI2 and ISCR1. The addition of 20 g/L biochar to cattle farm wastewater anaerobic digestion systems could reduce the environmental risk of ARGs.

https://www.sciencedirect.com/science/article/pii/S0960852418302360