摘要

本研究旨在使用全基因组测序（WGS）研究多重耐药（MDR）鲍曼不动杆菌（鲍氏不动杆菌）菌株MDR-SHH02中的抗生素抗性基因。使用Kirby-Bauer方法测定从乳腺癌患者中分离出的MDR-SHH02对19种抗生素的抗生素抗性。然后进行MDR-SHH02的WGS。在质量控制和转录组装之后，进行基因的功能注释，并且使用PHYLIP 3.695和FigTree v1.4.2构建MDR-SHH02的系统发生树，以及另外5个鲍曼不动杆菌物种和2个不动杆菌物种。此外，通过致病岛数据库预测致病岛（PAI）。基于抗生素抗性基因数据库（ARDB）中的信息预测MDR-SHH02中潜在的抗生素抗性基因。发现MDR-SHH02对所有测试的抗生素都具有抗性。 MDR-SHH02基因组总草图长度为4,003,808bp。有74.25％的编码序列被注释到21个蛋白质术语的同源基因簇（COG）中，如'转录'和'氨基酸转运和代谢'。此外，有45个PAI与序列MDRSHH02000806同源。此外，MDR-SHH02中的总共12个基因序列与ARDB中的抗生素抗性基因的序列高度相似，包括编码氨基糖苷修饰酶[例如，aac（3）-Ia，ant（2“） - Ia ，aph33ib和aph（3'） - Ia]，β-内酰胺酶基因（bl2b_tem和bl2b_tem1），磺胺抗性二氢蝶酸合成酶基因（sul1和sul2），catb3和tetb。这些结果表明，许多基因介导对MDR-SHH02中各种抗生素的耐药性，并且为鲍曼不动杆菌感染患者的个体化治疗提供临床指导。

This study aimed to investigate antibiotic resistance genes in the multidrug-resistant (MDR) Acinetobacter baumannii (A. baumanii) strain, MDR-SHH02, using wholegenome sequencing (WGS). The antibiotic resistance of MDR-SHH02 isolated from a patient with breast cancer to 19 types of antibiotics was determined using the KirbyBauer method. WGS of MDR-SHH02 was then performed. Following quality control and transcriptome assembly, functional annotation of genes was conducted, and the phylogenetic tree of MDR-SHH02, along with another 5 A. baumanii species and 2 Acinetobacter species, was constructed using PHYLIP 3.695 and FigTree v1.4.2. Furthermore, pathogenicity islands (PAIs) were predicted by the pathogenicity island database. Potential antibiotic resistance genes in MDR-SHH02 were predicted based on the information in the Antibiotic Resistance Genes Database (ARDB). MDR-SHH02 was found to be resistant to all of the tested antibiotics. The total draft genome length of MDR-SHH02 was 4,003,808 bp. There were 74.25% of coding sequences to be annotated into 21 of the Clusters of Orthologous Groups (COGs) of protein terms, such as ‘transcription’ and ‘amino acid transport and metabolism’. Furthermore, there were 45 PAIs homologous to the sequence MDRSHH02000806. Additionally, a total of 12 gene sequences in MDR-SHH02 were highly similar to the sequences of antibiotic resistance genes in ARDB, including genes encoding aminoglycosidemodifying enzymes [e.g., aac(3)-Ia, ant(2'')Ia, aph33ib and aph(3')-Ia], β-lactamase genes (bl2b_tem and bl2b_tem1), sulfonamide-resistant dihydropteroate synthase genes (sul1 and sul2), catb3 and tetb. These results suggest that numerous genes mediate resistance to various antibiotics in MDR-SHH02, and provide a clinical guidance for the personalized therapy of A. baumannii-infected patients.

https://www.spandidos-publications.com/10.3892/ijmm.2016.2844