摘要

生物电化学系统可以有效降解抗生素，但需要更好地了解抗生素生物电化学降解过程中抗生素耐药菌和抗生素耐药基因的命运。在这项研究中，BES被开发为一个平台，用于调查氯霉素生物降解期间不同操作条件下氯霉素抗性细菌（CRB）的命运和氯霉素抗性基因（CRG）的表达。结果表明，氯霉素浓度较低，阴极负极电位较高时，氯霉素被有效去除，氯霉素的去除率得到提高。较高的氯霉素浓度增加了CRB的富集和CRG的表达。此外，在更负的阴极电位下，CRB的丰度增加，诱导CRG在阴极负极电位较低时的表达。然而，CRB的富集和CRG的表达可以在中等阴极电位下缓和。该结果可为研究生物电化学系统抗生素耐药基因的命运提供科学参考。

Bioelectrochemical systems can effectively degrade antibiotics, but there is the need to better understand the fate of antibiotic resistance bacteria and antibiotic resistance genes during the bioelectrochemical degradation of antibiotics. In this study, a BES was developed as a platform to investigate the fate of chloramphenicol resistance bacteria (CRB) and the expression of chloramphenicol resistance genes (CRGs) under different operating conditions during chloramphenicol biodegradation. The results indicated that chloramphenicol was effectively removed and chloramphenicol removal efficiency could be improved under less chloramphenicol concentration and more negative cathode potential. Higher chloramphenicol concentration enhanced the enrichment of CRB and expression of CRGs. Furthermore, the abundances of CRB were enhanced under more negative cathode potential, the expression of CRGs under less negative cathode potential were induced. However, both the enrichment of CRB and expression of CRGs could be moderated under a medium cathode potential. This result could provide the scientific reference for research about the fate of antibiotic resistance genes in bioelectrochemical systems.

https://www.sciencedirect.com/science/article/pii/S004313541730252X